Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2

Renaud Dentin, Yi Liu, Seung-Hoi Koo, Susan Hedrick, Thomas Vargas, Jose Heredia, John Yates, Marc Montminy

Research output: Contribution to journalArticle

274 Citations (Scopus)

Abstract

During feeding, increases in circulating pancreatic insulin inhibit hepatic glucose output through the activation of the Ser/Thr kinase AKT and subsequent phosphorylation of the forkhead transcription factor FOXO1 (refs 1-3). Under fasting conditions, FOXO1 increases gluconeogenic gene expression in concert with the cAMP responsive coactivator TORC2 (refs 4-8). In response to pancreatic glucagon, TORC2 is de-phosphorylated at Ser 171 and transported to the nucleus, in which it stimulates the gluconeogenic programme by binding to CREB. Here we show in mice that insulin inhibits gluconeogenic gene expression during re-feeding by promoting the phosphorylation and ubiquitin-dependent degradation of TORC2. Insulin disrupts TORC2 activity by induction of the Ser/Thr kinase SIK2, which we show here undergoes AKT2-mediated phosphorylation at Ser 358. Activated SIK2 in turn stimulated the Ser 171 phosphorylation and cytoplasmic translocation of TORC2. Phosphorylated TORC2 was degraded by the 26S proteasome during re-feeding through an association with COP1, a substrate receptor for an E3 ligase complex that promoted TORC2 ubiquitination at Lys 628. Because TORC2 protein levels and activity were increased in diabetes owing to a block in TORC2 phosphorylation, our results point to an important role for this pathway in the maintenance of glucose homeostasis.

Original languageEnglish
Pages (from-to)366-369
Number of pages4
JournalNature
Volume449
Issue number7160
DOIs
Publication statusPublished - 2007 Sep 20
Externally publishedYes

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Gluconeogenesis
Insulin
Phosphorylation
Phosphotransferases
Forkhead Transcription Factors
Gene Expression
TOR complex 2
Inhibition (Psychology)
Glucose
Ubiquitin-Protein Ligases
Ubiquitination
Ubiquitin
Glucagon
Fasting
Homeostasis
Maintenance
Liver

ASJC Scopus subject areas

  • General

Cite this

Dentin, R., Liu, Y., Koo, S-H., Hedrick, S., Vargas, T., Heredia, J., ... Montminy, M. (2007). Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. Nature, 449(7160), 366-369. https://doi.org/10.1038/nature06128

Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. / Dentin, Renaud; Liu, Yi; Koo, Seung-Hoi; Hedrick, Susan; Vargas, Thomas; Heredia, Jose; Yates, John; Montminy, Marc.

In: Nature, Vol. 449, No. 7160, 20.09.2007, p. 366-369.

Research output: Contribution to journalArticle

Dentin, R, Liu, Y, Koo, S-H, Hedrick, S, Vargas, T, Heredia, J, Yates, J & Montminy, M 2007, 'Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2', Nature, vol. 449, no. 7160, pp. 366-369. https://doi.org/10.1038/nature06128
Dentin R, Liu Y, Koo S-H, Hedrick S, Vargas T, Heredia J et al. Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. Nature. 2007 Sep 20;449(7160):366-369. https://doi.org/10.1038/nature06128
Dentin, Renaud ; Liu, Yi ; Koo, Seung-Hoi ; Hedrick, Susan ; Vargas, Thomas ; Heredia, Jose ; Yates, John ; Montminy, Marc. / Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. In: Nature. 2007 ; Vol. 449, No. 7160. pp. 366-369.
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