Integration of electropreconcentration and electrospray ionization in a microchip

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3 Citations (Scopus)

Abstract

A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalJournal of Chromatography A
Volume1543
DOIs
Publication statusPublished - 2018 Mar 30

Keywords

  • Electropreconcentration
  • Electrospray ionization
  • Ion concentration polarization
  • Mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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