Abstract
A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
| Original language | English |
|---|---|
| Pages (from-to) | 67-72 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1543 |
| DOIs | |
| Publication status | Published - 2018 Mar 30 |
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Keywords
- Electropreconcentration
- Electrospray ionization
- Ion concentration polarization
- Mass spectrometry
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
Integration of electropreconcentration and electrospray ionization in a microchip. / Chun, Honggu.
In: Journal of Chromatography A, Vol. 1543, 30.03.2018, p. 67-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Integration of electropreconcentration and electrospray ionization in a microchip
AU - Chun, Honggu
PY - 2018/3/30
Y1 - 2018/3/30
N2 - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
AB - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
KW - Electropreconcentration
KW - Electrospray ionization
KW - Ion concentration polarization
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85042597050&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042597050&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2018.02.037
DO - 10.1016/j.chroma.2018.02.037
M3 - Article
VL - 1543
SP - 67
EP - 72
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
ER -