TY - JOUR
T1 - Integration of electropreconcentration and electrospray ionization in a microchip
AU - Chun, Honggu
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) ( NRF-2013M3A6A4046061 , NRF-2012-0009556 , and NRF-2011-0031866 ).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/3/30
Y1 - 2018/3/30
N2 - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
AB - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
KW - Electropreconcentration
KW - Electrospray ionization
KW - Ion concentration polarization
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85042597050&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2018.02.037
DO - 10.1016/j.chroma.2018.02.037
M3 - Article
C2 - 29501430
AN - SCOPUS:85042597050
VL - 1543
SP - 67
EP - 72
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -