Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

Jeongyun Kim, Juyeoul Baek, Hyughan Kim, Kyam Lee, Sang Hoon Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalSensors and Actuators, A: Physical
Volume128
Issue number1
DOIs
Publication statusPublished - 2006 Mar 31

Fingerprint

Immobilized Enzymes
Single-walled carbon nanotubes (SWCN)
Microfluidics
glucose
Glucose
enzymes
Enzymes
platforms
carbon nanotubes
Polydimethylsiloxane
microbalances
Glucose Oxidase
Glucose oxidase
Horseradish Peroxidase
microfluidic devices
oxidase
Fabrication
costs
fabrication
Costs

Keywords

  • Carbon nanotube
  • Glucose-detection
  • Microfluidics
  • PDMS

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Instrumentation

Cite this

Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection. / Kim, Jeongyun; Baek, Juyeoul; Kim, Hyughan; Lee, Kyam; Lee, Sang Hoon.

In: Sensors and Actuators, A: Physical, Vol. 128, No. 1, 31.03.2006, p. 7-13.

Research output: Contribution to journalArticle

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