Integration of Light- and Brassinosteroid-Signaling Pathways by a GATA Transcription Factor in Arabidopsis

Xiao Min Luo, Wen Hui Lin, Shengwei Zhu, Jia Ying Zhu, Yu Sun, Xi Ying Fan, Menglin Cheng, Yaqi Hao, Eunkyoo Oh, Miaomiao Tian, Lijing Liu, Ming Zhang, Qi Xie, Kang Chong, Zhi Yong Wang

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Light and brassinosteroid (BR) antagonistically regulate the developmental switch from etiolation in the dark to photomorphogenesis in the light in plants. Here, we identify GATA2 as a key transcriptional regulator that mediates the crosstalk between BR- and light-signaling pathways. Overexpression of GATA2 causes constitutive photomorphogenesis in the dark, whereas suppression of GATA2 reduces photomorphogenesis caused by light, BR deficiency, or the constitutive photomorphogenesis mutant cop1. Genome profiling and chromatin immunoprecipitation experiments show that GATA2 directly regulates genes that respond to both light and BR. BR represses GATA2 transcription through the BR-activated transcription factor BZR1, whereas light causes accumulation of GATA2 protein and feedback inhibition of GATA2 transcription. Dark-induced proteasomal degradation of GATA2 is dependent on the COP1 E3 ubiquitin ligase, and COP1 can ubiquitinate GATA2 in vitro. This study illustrates a molecular framework for antagonistic regulation of gene expression and seedling photomorphogenesis by BR and light.

Original languageEnglish
Pages (from-to)872-883
Number of pages12
JournalDevelopmental Cell
Volume19
Issue number6
DOIs
Publication statusPublished - 2010 Dec 14
Externally publishedYes

Fingerprint

Brassinosteroids
GATA Transcription Factors
Arabidopsis
Light
Transcription
Etiolation
Genes
Ubiquitin-Protein Ligases
Chromatin Immunoprecipitation
Gene Expression Regulation
Crosstalk
Seedlings
Gene expression
Chromatin
Transcription Factors
Switches
Genome
Feedback
Degradation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Integration of Light- and Brassinosteroid-Signaling Pathways by a GATA Transcription Factor in Arabidopsis. / Luo, Xiao Min; Lin, Wen Hui; Zhu, Shengwei; Zhu, Jia Ying; Sun, Yu; Fan, Xi Ying; Cheng, Menglin; Hao, Yaqi; Oh, Eunkyoo; Tian, Miaomiao; Liu, Lijing; Zhang, Ming; Xie, Qi; Chong, Kang; Wang, Zhi Yong.

In: Developmental Cell, Vol. 19, No. 6, 14.12.2010, p. 872-883.

Research output: Contribution to journalArticle

Luo, XM, Lin, WH, Zhu, S, Zhu, JY, Sun, Y, Fan, XY, Cheng, M, Hao, Y, Oh, E, Tian, M, Liu, L, Zhang, M, Xie, Q, Chong, K & Wang, ZY 2010, 'Integration of Light- and Brassinosteroid-Signaling Pathways by a GATA Transcription Factor in Arabidopsis', Developmental Cell, vol. 19, no. 6, pp. 872-883. https://doi.org/10.1016/j.devcel.2010.10.023
Luo, Xiao Min ; Lin, Wen Hui ; Zhu, Shengwei ; Zhu, Jia Ying ; Sun, Yu ; Fan, Xi Ying ; Cheng, Menglin ; Hao, Yaqi ; Oh, Eunkyoo ; Tian, Miaomiao ; Liu, Lijing ; Zhang, Ming ; Xie, Qi ; Chong, Kang ; Wang, Zhi Yong. / Integration of Light- and Brassinosteroid-Signaling Pathways by a GATA Transcription Factor in Arabidopsis. In: Developmental Cell. 2010 ; Vol. 19, No. 6. pp. 872-883.
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