Abstract
Background: Most human metabolomics studies have shown that spectral outputs of 1H nuclear magnetic resonance fingerprinting are strongly influenced by inter- and intra-individual variations; however, few studies have been performed to evaluate the inter- and intra-individual variations in urinary endogenous metabolites. Methods: We recruited 30 male college students to evaluate the factors affecting intra- and inter-individual variations in urinary endogenous metabolites. Statistical analysis for variations in urinary metabolites was performed after eliminating outliers found in principal component analysis (PCA) plots. Results: Inter-individual variations were relatively low for 2-oxoglutarate, succinate, citrate, dimethylglycine, and taurine, but high for trimethylaminoxide (TMAO), hippurate, and lactate. Intra-individual variations for 2-oxoglutarate, citrate, dimethylglycine, and taurine were relatively low, but high for TMAO and hippurate. The factors affecting inter-individual variation of lactate were age, body mass index, beverages, and alcohol, whereas the factors affecting intra-individual variation of lactate were age and fish. Kim Chi intake affected the inter-individual variation of succinate, citrate, TMAO, and hippurate; however, it did not affect the intra-individual variation of endogenous metabolites. Conclusions: Our results showed that inter- and intra-individual variations in urinary endogenous metabolites were very large, and significant factors affecting inter- and intra-individual variation were diverse, even after eliminating outliers in PCA analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 188-194 |
| Number of pages | 7 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 47 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Feb 1 |
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Keywords
- Genetic variation
- Magnetic resonance spectroscopy
- Metabolism
- Urine
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Inter- and intra-individual variations of urinary endogenous metabolites in healthy male college students using 1H NMR spectroscopy. / Mi Park, Eun; Lee, Eun Il; Jin Joo, Hyun; Oh, Eunha; Lee, Joohyun; Lee, Ji Sung.
In: Clinical Chemistry and Laboratory Medicine, Vol. 47, No. 2, 01.02.2009, p. 188-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inter- and intra-individual variations of urinary endogenous metabolites in healthy male college students using 1H NMR spectroscopy
AU - Mi Park, Eun
AU - Lee, Eun Il
AU - Jin Joo, Hyun
AU - Oh, Eunha
AU - Lee, Joohyun
AU - Lee, Ji Sung
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Background: Most human metabolomics studies have shown that spectral outputs of 1H nuclear magnetic resonance fingerprinting are strongly influenced by inter- and intra-individual variations; however, few studies have been performed to evaluate the inter- and intra-individual variations in urinary endogenous metabolites. Methods: We recruited 30 male college students to evaluate the factors affecting intra- and inter-individual variations in urinary endogenous metabolites. Statistical analysis for variations in urinary metabolites was performed after eliminating outliers found in principal component analysis (PCA) plots. Results: Inter-individual variations were relatively low for 2-oxoglutarate, succinate, citrate, dimethylglycine, and taurine, but high for trimethylaminoxide (TMAO), hippurate, and lactate. Intra-individual variations for 2-oxoglutarate, citrate, dimethylglycine, and taurine were relatively low, but high for TMAO and hippurate. The factors affecting inter-individual variation of lactate were age, body mass index, beverages, and alcohol, whereas the factors affecting intra-individual variation of lactate were age and fish. Kim Chi intake affected the inter-individual variation of succinate, citrate, TMAO, and hippurate; however, it did not affect the intra-individual variation of endogenous metabolites. Conclusions: Our results showed that inter- and intra-individual variations in urinary endogenous metabolites were very large, and significant factors affecting inter- and intra-individual variation were diverse, even after eliminating outliers in PCA analysis.
AB - Background: Most human metabolomics studies have shown that spectral outputs of 1H nuclear magnetic resonance fingerprinting are strongly influenced by inter- and intra-individual variations; however, few studies have been performed to evaluate the inter- and intra-individual variations in urinary endogenous metabolites. Methods: We recruited 30 male college students to evaluate the factors affecting intra- and inter-individual variations in urinary endogenous metabolites. Statistical analysis for variations in urinary metabolites was performed after eliminating outliers found in principal component analysis (PCA) plots. Results: Inter-individual variations were relatively low for 2-oxoglutarate, succinate, citrate, dimethylglycine, and taurine, but high for trimethylaminoxide (TMAO), hippurate, and lactate. Intra-individual variations for 2-oxoglutarate, citrate, dimethylglycine, and taurine were relatively low, but high for TMAO and hippurate. The factors affecting inter-individual variation of lactate were age, body mass index, beverages, and alcohol, whereas the factors affecting intra-individual variation of lactate were age and fish. Kim Chi intake affected the inter-individual variation of succinate, citrate, TMAO, and hippurate; however, it did not affect the intra-individual variation of endogenous metabolites. Conclusions: Our results showed that inter- and intra-individual variations in urinary endogenous metabolites were very large, and significant factors affecting inter- and intra-individual variation were diverse, even after eliminating outliers in PCA analysis.
KW - Genetic variation
KW - Magnetic resonance spectroscopy
KW - Metabolism
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=59849089923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59849089923&partnerID=8YFLogxK
U2 - 10.1515/CCLM.2009.040
DO - 10.1515/CCLM.2009.040
M3 - Article
VL - 47
SP - 188
EP - 194
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 2
ER -