Inter- and intra-individual variations of urinary endogenous metabolites in healthy male college students using 1H NMR spectroscopy

Eun Mi Park, Eunil Lee, Hyun Jin Joo, Eunha Oh, Joohyun Lee, Ji Sung Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)


Background: Most human metabolomics studies have shown that spectral outputs of 1H nuclear magnetic resonance fingerprinting are strongly influenced by inter- and intra-individual variations; however, few studies have been performed to evaluate the inter- and intra-individual variations in urinary endogenous metabolites. Methods: We recruited 30 male college students to evaluate the factors affecting intra- and inter-individual variations in urinary endogenous metabolites. Statistical analysis for variations in urinary metabolites was performed after eliminating outliers found in principal component analysis (PCA) plots. Results: Inter-individual variations were relatively low for 2-oxoglutarate, succinate, citrate, dimethylglycine, and taurine, but high for trimethylaminoxide (TMAO), hippurate, and lactate. Intra-individual variations for 2-oxoglutarate, citrate, dimethylglycine, and taurine were relatively low, but high for TMAO and hippurate. The factors affecting inter-individual variation of lactate were age, body mass index, beverages, and alcohol, whereas the factors affecting intra-individual variation of lactate were age and fish. Kim Chi intake affected the inter-individual variation of succinate, citrate, TMAO, and hippurate; however, it did not affect the intra-individual variation of endogenous metabolites. Conclusions: Our results showed that inter- and intra-individual variations in urinary endogenous metabolites were very large, and significant factors affecting inter- and intra-individual variation were diverse, even after eliminating outliers in PCA analysis.

Original languageEnglish
Pages (from-to)188-194
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Issue number2
Publication statusPublished - 2009 Feb 1



  • Genetic variation
  • Magnetic resonance spectroscopy
  • Metabolism
  • Urine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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