Inter- and intra-individual variations of urinary endogenous metabolites in healthy male college students using 1H NMR spectroscopy

Eun Mi Park, Eunil Lee, Hyun Jin Joo, Eunha Oh, Joohyun Lee, Ji Sung Lee

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9 Citations (Scopus)

Abstract

Background: Most human metabolomics studies have shown that spectral outputs of 1H nuclear magnetic resonance fingerprinting are strongly influenced by inter- and intra-individual variations; however, few studies have been performed to evaluate the inter- and intra-individual variations in urinary endogenous metabolites. Methods: We recruited 30 male college students to evaluate the factors affecting intra- and inter-individual variations in urinary endogenous metabolites. Statistical analysis for variations in urinary metabolites was performed after eliminating outliers found in principal component analysis (PCA) plots. Results: Inter-individual variations were relatively low for 2-oxoglutarate, succinate, citrate, dimethylglycine, and taurine, but high for trimethylaminoxide (TMAO), hippurate, and lactate. Intra-individual variations for 2-oxoglutarate, citrate, dimethylglycine, and taurine were relatively low, but high for TMAO and hippurate. The factors affecting inter-individual variation of lactate were age, body mass index, beverages, and alcohol, whereas the factors affecting intra-individual variation of lactate were age and fish. Kim Chi intake affected the inter-individual variation of succinate, citrate, TMAO, and hippurate; however, it did not affect the intra-individual variation of endogenous metabolites. Conclusions: Our results showed that inter- and intra-individual variations in urinary endogenous metabolites were very large, and significant factors affecting inter- and intra-individual variation were diverse, even after eliminating outliers in PCA analysis.

Original languageEnglish
Pages (from-to)188-194
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume47
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

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Keywords

  • Genetic variation
  • Magnetic resonance spectroscopy
  • Metabolism
  • Urine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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