Abstract
Yeast two-hybrid screening was conducted using a human ovary cDNA library to search for a novel binding protein using transforming growth factor-beta stimulated clone-22 (TSC-22). The selected protein was fortilin, which has been characterized as a nuclear anti-apoptotic protein. Overexpression of fortilin in ovarian carcinoma cells reversed TSC-22-mediated apoptosis, and the inhibition of fortilin expression via small interfering RNA (siRNA) resulted in an increase in the apoptosis of ovarian carcinoma cells. Moreover, fortilin overexpression promoted the degradation of TSC-22. Thus, an interaction between fortilin and TSC-22 prevents apoptosis via the destabilization of TSC-22 in ovarian carcinoma cells. Structured summary: MINT-6173230, MINT-6173253:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by co-immunoprecipitation (MI:0019)MINT-6173217:TSC22 (uniprotkb:Q15714) binds (MI:0407) fortilin (uniprotkb:P13693) by pull-down (MI:0096)MINT-6173240, MINT-6173270:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by two-hybrid (MI:0018).
| Original language | English |
|---|---|
| Pages (from-to) | 1210-1218 |
| Number of pages | 9 |
| Journal | FEBS Letters |
| Volume | 582 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2008 Apr 9 |
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Keywords
- Apoptosis
- Fortilin
- Ovarian carcinoma cell
- TSC-22
- Yeast two hybrid
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Interaction between fortilin and transforming growth factor-beta stimulated clone-22 (TSC-22) prevents apoptosis via the destabilization of TSC-22. / Lee, Jeong Heon; Rho, Seung Bae; Park, Sang Yoon; Chun, Taehoon.
In: FEBS Letters, Vol. 582, No. 8, 09.04.2008, p. 1210-1218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction between fortilin and transforming growth factor-beta stimulated clone-22 (TSC-22) prevents apoptosis via the destabilization of TSC-22
AU - Lee, Jeong Heon
AU - Rho, Seung Bae
AU - Park, Sang Yoon
AU - Chun, Taehoon
PY - 2008/4/9
Y1 - 2008/4/9
N2 - Yeast two-hybrid screening was conducted using a human ovary cDNA library to search for a novel binding protein using transforming growth factor-beta stimulated clone-22 (TSC-22). The selected protein was fortilin, which has been characterized as a nuclear anti-apoptotic protein. Overexpression of fortilin in ovarian carcinoma cells reversed TSC-22-mediated apoptosis, and the inhibition of fortilin expression via small interfering RNA (siRNA) resulted in an increase in the apoptosis of ovarian carcinoma cells. Moreover, fortilin overexpression promoted the degradation of TSC-22. Thus, an interaction between fortilin and TSC-22 prevents apoptosis via the destabilization of TSC-22 in ovarian carcinoma cells. Structured summary: MINT-6173230, MINT-6173253:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by co-immunoprecipitation (MI:0019)MINT-6173217:TSC22 (uniprotkb:Q15714) binds (MI:0407) fortilin (uniprotkb:P13693) by pull-down (MI:0096)MINT-6173240, MINT-6173270:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by two-hybrid (MI:0018).
AB - Yeast two-hybrid screening was conducted using a human ovary cDNA library to search for a novel binding protein using transforming growth factor-beta stimulated clone-22 (TSC-22). The selected protein was fortilin, which has been characterized as a nuclear anti-apoptotic protein. Overexpression of fortilin in ovarian carcinoma cells reversed TSC-22-mediated apoptosis, and the inhibition of fortilin expression via small interfering RNA (siRNA) resulted in an increase in the apoptosis of ovarian carcinoma cells. Moreover, fortilin overexpression promoted the degradation of TSC-22. Thus, an interaction between fortilin and TSC-22 prevents apoptosis via the destabilization of TSC-22 in ovarian carcinoma cells. Structured summary: MINT-6173230, MINT-6173253:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by co-immunoprecipitation (MI:0019)MINT-6173217:TSC22 (uniprotkb:Q15714) binds (MI:0407) fortilin (uniprotkb:P13693) by pull-down (MI:0096)MINT-6173240, MINT-6173270:TSC22 (uniprotkb:Q15714) physically interacts (MI:0218) with fortilin (uniprotkb:P13693) by two-hybrid (MI:0018).
KW - Apoptosis
KW - Fortilin
KW - Ovarian carcinoma cell
KW - TSC-22
KW - Yeast two hybrid
UR - http://www.scopus.com/inward/record.url?scp=41149096388&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41149096388&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2008.01.066
DO - 10.1016/j.febslet.2008.01.066
M3 - Article
C2 - 18325344
AN - SCOPUS:41149096388
VL - 582
SP - 1210
EP - 1218
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 8
ER -