Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation

Tae Sung Kim, Chang Young Jang, Hag Dong Kim, Jae Yung Lee, Byung-Yoon Ahn, Joon Kim

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Heat-shock protein 90 (Hsp90) is a molecular chaperone that plays a key role in the conformational maturation of various transcription factors and protein kinases in signal transduction. Multifunctional ribosomal protein S3 (rpS3), a component of the ribosomal small subunit, is involved in DNA repair and apoptosis. Our data show that Hsp90 binds directly to rpS3 and the functional consequence of Hsp90-rpS3 interaction results in the prevention of the ubiquitination and the proteasome-dependent degradation of rpS3, subsequently retaining the function and the biogenesis of the ribosome. Interference of Hsp90 activity by Hsp90 inhibitors appears to dissociate rpS3 from Hsp90, associate the protein with Hsp70, and induce the degradation of free forms of rpS3. Furthermore, ribosomal protein S6 (rpS6) also interacted with Hsp90 and exhibited a similar effect upon treatment with Hsp90 inhibitors. Therefore, we conclude that Hsp90 regulates the function of ribosomes by maintaining the stability of 40S ribosomal proteins such as rpS3 and rpS6.

Original languageEnglish
Pages (from-to)824-833
Number of pages10
JournalMolecular Biology of the Cell
Volume17
Issue number2
DOIs
Publication statusPublished - 2006 Feb 1

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HSP90 Heat-Shock Proteins
Ribosomal Proteins
Ubiquitination
Proteasome Endopeptidase Complex
Ribosomal Protein S6
Ribosomes
Small Ribosome Subunits
Molecular Chaperones
ribosomal protein S3
DNA Repair
Protein Kinases
Signal Transduction
Transcription Factors
Apoptosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation. / Kim, Tae Sung; Jang, Chang Young; Kim, Hag Dong; Lee, Jae Yung; Ahn, Byung-Yoon; Kim, Joon.

In: Molecular Biology of the Cell, Vol. 17, No. 2, 01.02.2006, p. 824-833.

Research output: Contribution to journalArticle

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AU - Kim, Joon

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