TY - JOUR
T1 - Interaction of NahR, a LysR-type transcriptional regulator, with the α subunit of RNA polymerase in the naphthalene degrading bacterium, Pseudomonas putida NCIB 9816-4
AU - Park, Woojun
AU - Jeon, Che Ok
AU - Madsen, Eugene L.
N1 - Funding Information:
This research was supported by National Science Foundation Grant MCB 0084175. Preliminary sequence data were obtained from The Institute for Genomic Research website at http://www.tigr.org . Sequences of P. putida KT2440 used here were the result of a DOE/BMBF project.
PY - 2002/8/6
Y1 - 2002/8/6
N2 - NahR, a LysR-type transcriptional regulator, is required for expression of naphthalene catabolic operons. However, detailed mechanisms of transcriptional activation by NahR are poorly understood. Many transcriptional activators make direct contact with RNA polymerase (RNAP) to initiate transcription. We investigated the hypothesis that direct contact between NahR and the α subunit of RNAP (αRNAP) may be involved in expression of the naphthalene catabolic operons in Pseudomonas putida NCIB 9816-4. Interactions between the NahR and αRNAP in P. putida NCIB 9816-4 were analyzed using the yeast two-hybrid system. The results obtained indicate that protein-protein interactions occur between αRNAP and the NahR. Gene activation by NahR is consistent with the general transcriptional mechanism of class I transcription factors, which function by contacting αRNAP.
AB - NahR, a LysR-type transcriptional regulator, is required for expression of naphthalene catabolic operons. However, detailed mechanisms of transcriptional activation by NahR are poorly understood. Many transcriptional activators make direct contact with RNA polymerase (RNAP) to initiate transcription. We investigated the hypothesis that direct contact between NahR and the α subunit of RNAP (αRNAP) may be involved in expression of the naphthalene catabolic operons in Pseudomonas putida NCIB 9816-4. Interactions between the NahR and αRNAP in P. putida NCIB 9816-4 were analyzed using the yeast two-hybrid system. The results obtained indicate that protein-protein interactions occur between αRNAP and the NahR. Gene activation by NahR is consistent with the general transcriptional mechanism of class I transcription factors, which function by contacting αRNAP.
KW - Protein-protein interaction
KW - RNA polymerase α subunit
KW - Transcriptional activation
KW - Yeast two-hybrid system
KW - rpoA sequence
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U2 - 10.1016/S0378-1097(02)00758-9
DO - 10.1016/S0378-1097(02)00758-9
M3 - Article
C2 - 12167532
AN - SCOPUS:0037031193
VL - 213
SP - 159
EP - 165
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -