Interaction of the Arabidopsis Receptor Protein Kinase Wak1 with a Glycine-rich Protein, AtGRP-3

Ae Ran Park, Somi K. Cho, Ui Jeong Yun, Mi Young Jin, Seoung Hyun Lee, Gilberto Sachetto-Martins, Ohkmae Kim

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

The Arabidopsis wall-associated receptor kinase, Wak1, is a member of the Wak family (Wak1-5) that links the plasma membrane to the extracellular matrix. By the yeast two-hybrid screen, we found that a glycine-rich extracellular protein, AtGRP-3, binds to the extracellular domain of Wak1. Further in vitro binding studies indicated that AtGRP-3 is the only isoform among the six tested AtGRPs that specifically interacts with Waks, and the cysteine-rich carboxyl terminus of AtGRP-3 is essential for its binding to Wak1. We also show that Wak1 and AtGRP-3 form a complex with a molecular size of -500 kDa in vivo in conjunction with the kinase-associated protein phosphatase, KAPP, that has been shown to interact with a number of plant receptor-like kinases. Binding of AtGRP-3 to Wak1 is shown to be crucial for the integrity of the complex. Wak1 and At-GRP-3 are both induced by salicylic acid treatment. Moreover, exogenously added AtGRP-3 up-regulates the expression of Wak1, AtGRP-3, and PR-1 (for pathogenesis-related) in protoplasts. Taken together, our data suggest that AtGRP-3 regulates Wak1 function through binding to the cell wall domain of Wak1 and that the interaction of Wak1 with AtGRP-3 occurs in a pathogenesis-related process in planta.

Original languageEnglish
Pages (from-to)26688-26693
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number28
DOIs
Publication statusPublished - 2001 Jul 13
Externally publishedYes

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Arabidopsis Proteins
Glycine
Protein Kinases
Phosphotransferases
Proteins
Salicylic Acid
Protoplasts
Phosphoprotein Phosphatases
Domain walls
Cell membranes
Arabidopsis
Yeast
Cell Wall
Extracellular Matrix
Cysteine
Protein Isoforms
Up-Regulation
Yeasts
Cells
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction of the Arabidopsis Receptor Protein Kinase Wak1 with a Glycine-rich Protein, AtGRP-3. / Park, Ae Ran; Cho, Somi K.; Yun, Ui Jeong; Jin, Mi Young; Lee, Seoung Hyun; Sachetto-Martins, Gilberto; Kim, Ohkmae.

In: Journal of Biological Chemistry, Vol. 276, No. 28, 13.07.2001, p. 26688-26693.

Research output: Contribution to journalArticle

Park, Ae Ran ; Cho, Somi K. ; Yun, Ui Jeong ; Jin, Mi Young ; Lee, Seoung Hyun ; Sachetto-Martins, Gilberto ; Kim, Ohkmae. / Interaction of the Arabidopsis Receptor Protein Kinase Wak1 with a Glycine-rich Protein, AtGRP-3. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 28. pp. 26688-26693.
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