Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation

Sang Eun Park, Seung Hun Jeong, Soo Bog Yee, Tae Hyun Kim, Young Hwa Soung, Nam Chul Ha, Nam Deuk Kim, Jae Yong Park, Hae Rahn Bae, Bong Soo Park, Hye Jeong Lee, Young Hyun Yoo

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24 Citations (Scopus)

Abstract

Acetylcholinesterase (AChE) is emerging as an important component in leading to apoptosis. Our previous study demonstrated that silencing of the AChE gene blocked the interaction between cytochrome c and apoptotic protease-activating factor-1 (Apaf-1) in etoposide-induced apoptosis of HT-29 cells. We undertook this study to further dissect the molecular role of AChE in apoptosome formation. The present study elicited that small interfering RNA (siRNA) to cytochrome c gene blocked the interaction of AChE with Apaf-1, whereas siRNA to Apaf-1 gene did not block the interaction of AChE with cytochrome c, indicating that the interaction of AChE with cytochrome c is required for the interaction between cytochrome c and protease-activating factor-1. We further observed that AChE is localized to caveolae via interacting with caveolin-1 during apoptosis and that the disruption of caveolae prevented apoptosome formation. These data indicate that the interactions of AChE with caveolin-1 and subsequently with cytochrome c appear to be indispensable for apoptosome formation.

Original languageEnglish
Pages (from-to)729-737
Number of pages9
JournalCarcinogenesis
Volume29
Issue number4
DOIs
Publication statusPublished - 2008 Apr

ASJC Scopus subject areas

  • Cancer Research

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    Park, S. E., Jeong, S. H., Yee, S. B., Kim, T. H., Soung, Y. H., Ha, N. C., Kim, N. D., Park, J. Y., Bae, H. R., Park, B. S., Lee, H. J., & Yoo, Y. H. (2008). Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation. Carcinogenesis, 29(4), 729-737. https://doi.org/10.1093/carcin/bgn036