Interactive transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157

H7 and intestinal epithelial HT-29 cells after bacterial attachment

Younghoon Kim, Sejong Oh, Sungsu Park, Sae Hun Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Here, the gene expression profiles of EHEC O157:H7 and HT-29 during the attachment stage were investigated by using duplex whole transcriptome analysis. After the initial attachment (3 h), the gene regulation systems of both the EHEC O157:H7 and HT-29 host cells were immediately remodeled. A total of 326 genes of the HT-29 cells, which involved proteins associated with the detoxification process, stress response proteins, anti-apoptosis/inflammation proteins, immune response protein, and oxidative stress proteins, were differentially regulated by more than 2.0-fold during EHEC attachment. In contrast, when HT-29 was attached to EHEC the expression of 611 genes was induced and the expression of 384 genes was reduced by more than twofold when compared to RPMI 1640-grown EHEC (16.14% of the total hybridized genes). Among the genes that were classified according to biological function, the mRNA levels of the genes involved in stress response, oxidative stress, cell signaling and cell surface proteins were significantly altered after the attachment of EHEC O157:H7. Therefore, the results of this study provide crucial insight into the genetic networks that provide host cell protection and the strategy of EHEC O157:H7 pathogenesis in gastro-intestinal (GI) tracts.

Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalInternational Journal of Food Microbiology
Volume131
Issue number2-3
DOIs
Publication statusPublished - 2009 May 31

Fingerprint

Enterohemorrhagic Escherichia coli
enterohemorrhagic Escherichia coli
HT29 Cells
Escherichia coli O157
Gene Expression Profiling
transcriptomics
Escherichia coli
Epithelial Cells
Genes
Proteins
cells
Oxidative stress
Heat-Shock Proteins
genes
Gene expression
proteins
gene expression
stress response
Oxidative Stress
oxidative stress

Keywords

  • Attachment
  • EHEC O157:H7
  • Host cell
  • Transcriptomic analysis

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

Interactive transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157 : H7 and intestinal epithelial HT-29 cells after bacterial attachment. / Kim, Younghoon; Oh, Sejong; Park, Sungsu; Kim, Sae Hun.

In: International Journal of Food Microbiology, Vol. 131, No. 2-3, 31.05.2009, p. 224-232.

Research output: Contribution to journalArticle

@article{02c0bb51c414417f8dcac203cc47244e,
title = "Interactive transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157: H7 and intestinal epithelial HT-29 cells after bacterial attachment",
abstract = "Here, the gene expression profiles of EHEC O157:H7 and HT-29 during the attachment stage were investigated by using duplex whole transcriptome analysis. After the initial attachment (3 h), the gene regulation systems of both the EHEC O157:H7 and HT-29 host cells were immediately remodeled. A total of 326 genes of the HT-29 cells, which involved proteins associated with the detoxification process, stress response proteins, anti-apoptosis/inflammation proteins, immune response protein, and oxidative stress proteins, were differentially regulated by more than 2.0-fold during EHEC attachment. In contrast, when HT-29 was attached to EHEC the expression of 611 genes was induced and the expression of 384 genes was reduced by more than twofold when compared to RPMI 1640-grown EHEC (16.14{\%} of the total hybridized genes). Among the genes that were classified according to biological function, the mRNA levels of the genes involved in stress response, oxidative stress, cell signaling and cell surface proteins were significantly altered after the attachment of EHEC O157:H7. Therefore, the results of this study provide crucial insight into the genetic networks that provide host cell protection and the strategy of EHEC O157:H7 pathogenesis in gastro-intestinal (GI) tracts.",
keywords = "Attachment, EHEC O157:H7, Host cell, Transcriptomic analysis",
author = "Younghoon Kim and Sejong Oh and Sungsu Park and Kim, {Sae Hun}",
year = "2009",
month = "5",
day = "31",
doi = "10.1016/j.ijfoodmicro.2009.03.002",
language = "English",
volume = "131",
pages = "224--232",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Interactive transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157

T2 - H7 and intestinal epithelial HT-29 cells after bacterial attachment

AU - Kim, Younghoon

AU - Oh, Sejong

AU - Park, Sungsu

AU - Kim, Sae Hun

PY - 2009/5/31

Y1 - 2009/5/31

N2 - Here, the gene expression profiles of EHEC O157:H7 and HT-29 during the attachment stage were investigated by using duplex whole transcriptome analysis. After the initial attachment (3 h), the gene regulation systems of both the EHEC O157:H7 and HT-29 host cells were immediately remodeled. A total of 326 genes of the HT-29 cells, which involved proteins associated with the detoxification process, stress response proteins, anti-apoptosis/inflammation proteins, immune response protein, and oxidative stress proteins, were differentially regulated by more than 2.0-fold during EHEC attachment. In contrast, when HT-29 was attached to EHEC the expression of 611 genes was induced and the expression of 384 genes was reduced by more than twofold when compared to RPMI 1640-grown EHEC (16.14% of the total hybridized genes). Among the genes that were classified according to biological function, the mRNA levels of the genes involved in stress response, oxidative stress, cell signaling and cell surface proteins were significantly altered after the attachment of EHEC O157:H7. Therefore, the results of this study provide crucial insight into the genetic networks that provide host cell protection and the strategy of EHEC O157:H7 pathogenesis in gastro-intestinal (GI) tracts.

AB - Here, the gene expression profiles of EHEC O157:H7 and HT-29 during the attachment stage were investigated by using duplex whole transcriptome analysis. After the initial attachment (3 h), the gene regulation systems of both the EHEC O157:H7 and HT-29 host cells were immediately remodeled. A total of 326 genes of the HT-29 cells, which involved proteins associated with the detoxification process, stress response proteins, anti-apoptosis/inflammation proteins, immune response protein, and oxidative stress proteins, were differentially regulated by more than 2.0-fold during EHEC attachment. In contrast, when HT-29 was attached to EHEC the expression of 611 genes was induced and the expression of 384 genes was reduced by more than twofold when compared to RPMI 1640-grown EHEC (16.14% of the total hybridized genes). Among the genes that were classified according to biological function, the mRNA levels of the genes involved in stress response, oxidative stress, cell signaling and cell surface proteins were significantly altered after the attachment of EHEC O157:H7. Therefore, the results of this study provide crucial insight into the genetic networks that provide host cell protection and the strategy of EHEC O157:H7 pathogenesis in gastro-intestinal (GI) tracts.

KW - Attachment

KW - EHEC O157:H7

KW - Host cell

KW - Transcriptomic analysis

UR - http://www.scopus.com/inward/record.url?scp=64349097094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64349097094&partnerID=8YFLogxK

U2 - 10.1016/j.ijfoodmicro.2009.03.002

DO - 10.1016/j.ijfoodmicro.2009.03.002

M3 - Article

VL - 131

SP - 224

EP - 232

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 2-3

ER -