Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee, Kwang Hee Bae, Chang Won Kho, Sunghyun Kang, Do Hee Lee, Sayeon Cho, Seong Man Kang, Sang Chul Lee, Byoung Chul Park, Sung Goo Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

Original languageEnglish
Pages (from-to)1364-1367
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume18
Issue number8
Publication statusPublished - 2008 Aug 28

Fingerprint

Glutathione Peroxidase
Yeasts
Oxidative Stress
Proteins
Saccharomyces cerevisiae
Glutaredoxins
Protein Disulfide-Isomerases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Immunoprecipitation
Oxidation-Reduction
Mass Spectrometry

Keywords

  • Glutathione peroxidase 3
  • IP-2DE
  • Oxidative stress
  • ROS

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lee, P. Y., Bae, K. H., Kho, C. W., Kang, S., Lee, D. H., Cho, S., ... Park, S. G. (2008). Interactome analysis of yeast glutathione peroxidase 3. Journal of Microbiology and Biotechnology, 18(8), 1364-1367.

Interactome analysis of yeast glutathione peroxidase 3. / Lee, Phil Young; Bae, Kwang Hee; Kho, Chang Won; Kang, Sunghyun; Lee, Do Hee; Cho, Sayeon; Kang, Seong Man; Lee, Sang Chul; Park, Byoung Chul; Park, Sung Goo.

In: Journal of Microbiology and Biotechnology, Vol. 18, No. 8, 28.08.2008, p. 1364-1367.

Research output: Contribution to journalArticle

Lee, PY, Bae, KH, Kho, CW, Kang, S, Lee, DH, Cho, S, Kang, SM, Lee, SC, Park, BC & Park, SG 2008, 'Interactome analysis of yeast glutathione peroxidase 3', Journal of Microbiology and Biotechnology, vol. 18, no. 8, pp. 1364-1367.
Lee PY, Bae KH, Kho CW, Kang S, Lee DH, Cho S et al. Interactome analysis of yeast glutathione peroxidase 3. Journal of Microbiology and Biotechnology. 2008 Aug 28;18(8):1364-1367.
Lee, Phil Young ; Bae, Kwang Hee ; Kho, Chang Won ; Kang, Sunghyun ; Lee, Do Hee ; Cho, Sayeon ; Kang, Seong Man ; Lee, Sang Chul ; Park, Byoung Chul ; Park, Sung Goo. / Interactome analysis of yeast glutathione peroxidase 3. In: Journal of Microbiology and Biotechnology. 2008 ; Vol. 18, No. 8. pp. 1364-1367.
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