Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee, Kwang Hee Bae, Chang Won Kho, Sunghyun Kang, Do Hee Lee, Sayeon Cho, Seongman Kang, Sang Chul Lee, Byoung Chul Park, Sung Goo Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

Original languageEnglish
Pages (from-to)1364-1367
Number of pages4
JournalJournal of microbiology and biotechnology
Volume18
Issue number8
Publication statusPublished - 2008 Aug 28

Keywords

  • Glutathione peroxidase 3
  • IP-2DE
  • Oxidative stress
  • ROS

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Lee, P. Y., Bae, K. H., Kho, C. W., Kang, S., Lee, D. H., Cho, S., Kang, S., Lee, S. C., Park, B. C., & Park, S. G. (2008). Interactome analysis of yeast glutathione peroxidase 3. Journal of microbiology and biotechnology, 18(8), 1364-1367.