Intercellular interaction observed by atomic force microscopy

Su Jin Kim, Sejin Kim, Hyunjung Shin, Chang Sub Uhm

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    The cultured myoblasts, focusing on the microprocesses related to the intercellular interaction, were observed by taking topological images. For atomic force microscopy (AFM), cells were fixed and either dried as in usual scanning electron microscopy or kept in the buffer. The dried cells were used for observing intercellular interactions related to the fusion. The prefusing myoblasts aligned in a chain were mostly spindle in shape and were characterized by the presence of many microprocesses along the facing edges of adjacent aligned myoblasts. The space between fusing myoblasts and between myotubes and myoblasts were often traversed by filopodia and cellular bridges formed by the connection of microvilli. These results suggest that microprocesses may be involved in the fusion of myoblasts. The best images of the fixed cell in liquid were obtained using the contact mode of AFM. AFM observation is an efficient tool in the study on the interaction between cells, and the fixation, imaging in liquid is a good approach to understand the cellular dynamics.

    Original languageEnglish
    Pages (from-to)1148-1151
    Number of pages4
    JournalUltramicroscopy
    Volume108
    Issue number10
    DOIs
    Publication statusPublished - 2008 Sep

    Keywords

    • Biochips
    • Biological materials
    • Cellular interaction
    • Culture
    • Nanostructures
    • Scanning probe microscopy
    • Sensors

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Atomic and Molecular Physics, and Optics
    • Instrumentation

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