Intercellular interaction observed by atomic force microscopy

Su Jin Kim, Sejin Kim, Hyunjung Shin, Chang Sub Uhm

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The cultured myoblasts, focusing on the microprocesses related to the intercellular interaction, were observed by taking topological images. For atomic force microscopy (AFM), cells were fixed and either dried as in usual scanning electron microscopy or kept in the buffer. The dried cells were used for observing intercellular interactions related to the fusion. The prefusing myoblasts aligned in a chain were mostly spindle in shape and were characterized by the presence of many microprocesses along the facing edges of adjacent aligned myoblasts. The space between fusing myoblasts and between myotubes and myoblasts were often traversed by filopodia and cellular bridges formed by the connection of microvilli. These results suggest that microprocesses may be involved in the fusion of myoblasts. The best images of the fixed cell in liquid were obtained using the contact mode of AFM. AFM observation is an efficient tool in the study on the interaction between cells, and the fixation, imaging in liquid is a good approach to understand the cellular dynamics.

Original languageEnglish
Pages (from-to)1148-1151
Number of pages4
JournalUltramicroscopy
Volume108
Issue number10
DOIs
Publication statusPublished - 2008 Sep 1

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Keywords

  • Biochips
  • Biological materials
  • Cellular interaction
  • Culture
  • Nanostructures
  • Scanning probe microscopy
  • Sensors

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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