Abstract
Ion-sensitive field-effect transistor (ISFET) as a biosensor facilitates a process of data-acquisition through label-free and real-time monitoring. Direct quantification of a biomarker in serum is challenging in ISFET biosensor since charged proteins in serum interfere transduction to electrical signals. Here, we report the fabrication of protein blocking layers (PBLs) with intended interfacial charges to minimize non-specific protein bindings on ISFET. Use of charged protein precursors enables to regulate the interfacial charge of PBLs, preserving their intrinsic electric features (neutral: hemoglobin, positively charged: lysozyme, negatively charged: BSA). The effect of this interfacial charge on the signal was demonstrated through PSMA (prostate cancer biomarker) sensing using a dual-gate ISFET biosensor. The neutral PBL showed the minimum noise compared to the negatively and positively charged PBLs, enabling the ISFET to exhibit the same detection range in untreated serum as with pre- or post-treatment (1 fg/ml to 100 ng/ml). The introduction of neutral PBLs to ISFET biosensors would allow the application of the ISFET biosensor as a point-of-care device.
| Original language | English |
|---|---|
| Article number | 111737 |
| Journal | Biosensors and Bioelectronics |
| Volume | 147 |
| DOIs | |
| Publication status | Published - 2020 Jan 1 |
Keywords
- Biosensor
- Field-effect transistor
- Non-specific binding
- Protein blocking layer
- Serum
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
Interfacial charge regulation of protein blocking layers in transistor biosensor for direct measurement in serum. / Park, Sungwook; Kim, Minsoo; Kim, Dongkap; Kang, Seok Ho; Lee, Kwan Hyi; Jeong, Youngdo.
In: Biosensors and Bioelectronics, Vol. 147, 111737, 01.01.2020.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interfacial charge regulation of protein blocking layers in transistor biosensor for direct measurement in serum
AU - Park, Sungwook
AU - Kim, Minsoo
AU - Kim, Dongkap
AU - Kang, Seok Ho
AU - Lee, Kwan Hyi
AU - Jeong, Youngdo
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Ion-sensitive field-effect transistor (ISFET) as a biosensor facilitates a process of data-acquisition through label-free and real-time monitoring. Direct quantification of a biomarker in serum is challenging in ISFET biosensor since charged proteins in serum interfere transduction to electrical signals. Here, we report the fabrication of protein blocking layers (PBLs) with intended interfacial charges to minimize non-specific protein bindings on ISFET. Use of charged protein precursors enables to regulate the interfacial charge of PBLs, preserving their intrinsic electric features (neutral: hemoglobin, positively charged: lysozyme, negatively charged: BSA). The effect of this interfacial charge on the signal was demonstrated through PSMA (prostate cancer biomarker) sensing using a dual-gate ISFET biosensor. The neutral PBL showed the minimum noise compared to the negatively and positively charged PBLs, enabling the ISFET to exhibit the same detection range in untreated serum as with pre- or post-treatment (1 fg/ml to 100 ng/ml). The introduction of neutral PBLs to ISFET biosensors would allow the application of the ISFET biosensor as a point-of-care device.
AB - Ion-sensitive field-effect transistor (ISFET) as a biosensor facilitates a process of data-acquisition through label-free and real-time monitoring. Direct quantification of a biomarker in serum is challenging in ISFET biosensor since charged proteins in serum interfere transduction to electrical signals. Here, we report the fabrication of protein blocking layers (PBLs) with intended interfacial charges to minimize non-specific protein bindings on ISFET. Use of charged protein precursors enables to regulate the interfacial charge of PBLs, preserving their intrinsic electric features (neutral: hemoglobin, positively charged: lysozyme, negatively charged: BSA). The effect of this interfacial charge on the signal was demonstrated through PSMA (prostate cancer biomarker) sensing using a dual-gate ISFET biosensor. The neutral PBL showed the minimum noise compared to the negatively and positively charged PBLs, enabling the ISFET to exhibit the same detection range in untreated serum as with pre- or post-treatment (1 fg/ml to 100 ng/ml). The introduction of neutral PBLs to ISFET biosensors would allow the application of the ISFET biosensor as a point-of-care device.
KW - Biosensor
KW - Field-effect transistor
KW - Non-specific binding
KW - Protein blocking layer
KW - Serum
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UR - http://www.scopus.com/inward/citedby.url?scp=85073682790&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2019.111737
DO - 10.1016/j.bios.2019.111737
M3 - Article
C2 - 31655380
AN - SCOPUS:85073682790
VL - 147
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
M1 - 111737
ER -