Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs

Jeong Heon Lee, Taehoon Chun, Sang Yoon Park, Seung Bae Rho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Interferon regulatory factor-1 (IRF-1) is a tumor suppressor and transcriptional modulator that can regulate gene expression involved in cell growth control, induction of apoptosis, and post-translation modification. In this study, we found that IRF-1 inhibits endothelial cell angiogenesis using human umbilical vein endothelial cell (HUVECs) culture system. In addition, IRF-1 directly inhibited the tube formation of endothelial cells on Matrigel and reduced the expression of p-Akt, and p-eNOS, which play a significant role in angiogenesis when stimulated by VEGF. We also demonstrate that C-terminal region including transactivation domain (TA) of IRF-1 functions as a signal for its angiostatic activity, and is spliced in human tumor tissues. These findings indicate that splicing variant involving exons 7 of IRF-1 could potentially modulate anti-angiogenic effect of IRF-1. In overall, this study provides the first evidence for anti-angiogenic role of IRF-1, which may have therapeutic values for cancer and angiogenesis-associated diseases.

Original languageEnglish
Pages (from-to)1654-1662
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1783
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Interferon Regulatory Factor-1
Human Umbilical Vein Endothelial Cells
Vascular Endothelial Growth Factor A
Endothelial Cells
Neoplasms
Transcriptional Activation
Exons
Cell Culture Techniques
Apoptosis
Gene Expression
Growth

Keywords

  • Alternative splicing
  • Angiogenesis
  • CAM
  • Endothelial cell
  • Interferon regulatory factor
  • Vessel sprouting

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics
  • Medicine(all)

Cite this

Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs. / Lee, Jeong Heon; Chun, Taehoon; Park, Sang Yoon; Rho, Seung Bae.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1783, No. 9, 01.09.2008, p. 1654-1662.

Research output: Contribution to journalArticle

@article{662046806e0847e2901e5f774b27241c,
title = "Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs",
abstract = "Interferon regulatory factor-1 (IRF-1) is a tumor suppressor and transcriptional modulator that can regulate gene expression involved in cell growth control, induction of apoptosis, and post-translation modification. In this study, we found that IRF-1 inhibits endothelial cell angiogenesis using human umbilical vein endothelial cell (HUVECs) culture system. In addition, IRF-1 directly inhibited the tube formation of endothelial cells on Matrigel and reduced the expression of p-Akt, and p-eNOS, which play a significant role in angiogenesis when stimulated by VEGF. We also demonstrate that C-terminal region including transactivation domain (TA) of IRF-1 functions as a signal for its angiostatic activity, and is spliced in human tumor tissues. These findings indicate that splicing variant involving exons 7 of IRF-1 could potentially modulate anti-angiogenic effect of IRF-1. In overall, this study provides the first evidence for anti-angiogenic role of IRF-1, which may have therapeutic values for cancer and angiogenesis-associated diseases.",
keywords = "Alternative splicing, Angiogenesis, CAM, Endothelial cell, Interferon regulatory factor, Vessel sprouting",
author = "Lee, {Jeong Heon} and Taehoon Chun and Park, {Sang Yoon} and Rho, {Seung Bae}",
year = "2008",
month = "9",
day = "1",
doi = "10.1016/j.bbamcr.2008.04.006",
language = "English",
volume = "1783",
pages = "1654--1662",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - Interferon regulatory factor-1 (IRF-1) regulates VEGF-induced angiogenesis in HUVECs

AU - Lee, Jeong Heon

AU - Chun, Taehoon

AU - Park, Sang Yoon

AU - Rho, Seung Bae

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Interferon regulatory factor-1 (IRF-1) is a tumor suppressor and transcriptional modulator that can regulate gene expression involved in cell growth control, induction of apoptosis, and post-translation modification. In this study, we found that IRF-1 inhibits endothelial cell angiogenesis using human umbilical vein endothelial cell (HUVECs) culture system. In addition, IRF-1 directly inhibited the tube formation of endothelial cells on Matrigel and reduced the expression of p-Akt, and p-eNOS, which play a significant role in angiogenesis when stimulated by VEGF. We also demonstrate that C-terminal region including transactivation domain (TA) of IRF-1 functions as a signal for its angiostatic activity, and is spliced in human tumor tissues. These findings indicate that splicing variant involving exons 7 of IRF-1 could potentially modulate anti-angiogenic effect of IRF-1. In overall, this study provides the first evidence for anti-angiogenic role of IRF-1, which may have therapeutic values for cancer and angiogenesis-associated diseases.

AB - Interferon regulatory factor-1 (IRF-1) is a tumor suppressor and transcriptional modulator that can regulate gene expression involved in cell growth control, induction of apoptosis, and post-translation modification. In this study, we found that IRF-1 inhibits endothelial cell angiogenesis using human umbilical vein endothelial cell (HUVECs) culture system. In addition, IRF-1 directly inhibited the tube formation of endothelial cells on Matrigel and reduced the expression of p-Akt, and p-eNOS, which play a significant role in angiogenesis when stimulated by VEGF. We also demonstrate that C-terminal region including transactivation domain (TA) of IRF-1 functions as a signal for its angiostatic activity, and is spliced in human tumor tissues. These findings indicate that splicing variant involving exons 7 of IRF-1 could potentially modulate anti-angiogenic effect of IRF-1. In overall, this study provides the first evidence for anti-angiogenic role of IRF-1, which may have therapeutic values for cancer and angiogenesis-associated diseases.

KW - Alternative splicing

KW - Angiogenesis

KW - CAM

KW - Endothelial cell

KW - Interferon regulatory factor

KW - Vessel sprouting

UR - http://www.scopus.com/inward/record.url?scp=49149119083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49149119083&partnerID=8YFLogxK

U2 - 10.1016/j.bbamcr.2008.04.006

DO - 10.1016/j.bbamcr.2008.04.006

M3 - Article

VL - 1783

SP - 1654

EP - 1662

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 9

ER -