Interleukin-12 p40 gene expression is induced in lipopolysaccharide-activated pituitary glands in vivo

Young Yang, Seung Hyun Han, Hyun Kim, Changmee Kim, Kun Yong Kim, Sun Mi Shin, Inpyo Choi, Kwang Ho Pyun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Proinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-κB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production. Copyright copy; 2002 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)347-357
Number of pages11
JournalNeuroendocrinology
Volume75
Issue number6
DOIs
Publication statusPublished - 2002 Jun 26

    Fingerprint

Keywords

  • Catecholamine agonists
  • Hypothalamo-pituitary-adrenal axis
  • Interleukins
  • Lipopolysaccharide
  • Neuroimmune interactions

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Yang, Y., Han, S. H., Kim, H., Kim, C., Kim, K. Y., Shin, S. M., Choi, I., & Pyun, K. H. (2002). Interleukin-12 p40 gene expression is induced in lipopolysaccharide-activated pituitary glands in vivo. Neuroendocrinology, 75(6), 347-357. https://doi.org/10.1159/000059431