Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells

Ha reum Lee, Sun Young Yoon, Seok Bean Song, Yoorim Park, Tae Sung Kim, Seonghan Kim, Dae Young Hur, Hyun Keun Song, Hyunjeong Park, Daeho Cho

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.

Original languageEnglish
Pages (from-to)1155-1162
Number of pages8
JournalImmunobiology
Volume216
Issue number10
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • Interferon-gamma
  • Interleukin-18
  • Natural killer cells
  • Thymosin beta 4

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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