Abstract
Recent studies have reported that neuronal apoptosis is induced not only by extracellular Aβ but also by intracellular Aβ; however, the mechanism by which intracellular Aβ contributes to the regulation of cell death associated with the pathogenesis of AD remains to be elucidated. Using immunological assays and a short-lived enhanced green fluorescent protein (d2EGFP) system, we showed that intracellular Aβ and C99 form perinuclear aggregates in the cytosol, and the resulting aggregates attenuate the activity of the 26S proteasome. In addition, the immunofluorescence assays (IFA) revealed that the 20S proteasome α-subunits are recruited into perinuclear aggregates in both human embryonic kidney (HEK293) and human neuroglioma H4 (H4) cells. Interestingly, we observed an increase in the levels of Bax, cleavage of PARP-1, and mitochondrial release of proapoptotic proteins, such as cytochrome c and HtrA2, in H4 cells with intracellular Aβ or C99 aggregates, but not in HEK293 cells with those aggregates. The results of the present study indicate that intracellular Aβ and C99 aggregates induce mitochondria-dependent apoptotic cell death via elevation of Bax levels as a result of proteasome inhibition in a cell type-specific manner.
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 1273 |
| DOIs | |
| Publication status | Published - 2009 Jun 1 |
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Keywords
- Alzheimer's disease
- Amyloid beta (Aβ)
- C99
- Human neuroglioma H4 cell
- Mitochondria-dependent cell death
- Proteasome
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Intracellular Aβ and C99 aggregates induce mitochondria-dependent cell death in human neuroglioma H4 cells through recruitment of the 20S proteasome subunits. / Park, Hyo Jin; Kim, Sang Soo; Kang, Seong Man; Rhim, Hyangshuk.
In: Brain Research, Vol. 1273, 01.06.2009, p. 1-8.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intracellular Aβ and C99 aggregates induce mitochondria-dependent cell death in human neuroglioma H4 cells through recruitment of the 20S proteasome subunits
AU - Park, Hyo Jin
AU - Kim, Sang Soo
AU - Kang, Seong Man
AU - Rhim, Hyangshuk
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Recent studies have reported that neuronal apoptosis is induced not only by extracellular Aβ but also by intracellular Aβ; however, the mechanism by which intracellular Aβ contributes to the regulation of cell death associated with the pathogenesis of AD remains to be elucidated. Using immunological assays and a short-lived enhanced green fluorescent protein (d2EGFP) system, we showed that intracellular Aβ and C99 form perinuclear aggregates in the cytosol, and the resulting aggregates attenuate the activity of the 26S proteasome. In addition, the immunofluorescence assays (IFA) revealed that the 20S proteasome α-subunits are recruited into perinuclear aggregates in both human embryonic kidney (HEK293) and human neuroglioma H4 (H4) cells. Interestingly, we observed an increase in the levels of Bax, cleavage of PARP-1, and mitochondrial release of proapoptotic proteins, such as cytochrome c and HtrA2, in H4 cells with intracellular Aβ or C99 aggregates, but not in HEK293 cells with those aggregates. The results of the present study indicate that intracellular Aβ and C99 aggregates induce mitochondria-dependent apoptotic cell death via elevation of Bax levels as a result of proteasome inhibition in a cell type-specific manner.
AB - Recent studies have reported that neuronal apoptosis is induced not only by extracellular Aβ but also by intracellular Aβ; however, the mechanism by which intracellular Aβ contributes to the regulation of cell death associated with the pathogenesis of AD remains to be elucidated. Using immunological assays and a short-lived enhanced green fluorescent protein (d2EGFP) system, we showed that intracellular Aβ and C99 form perinuclear aggregates in the cytosol, and the resulting aggregates attenuate the activity of the 26S proteasome. In addition, the immunofluorescence assays (IFA) revealed that the 20S proteasome α-subunits are recruited into perinuclear aggregates in both human embryonic kidney (HEK293) and human neuroglioma H4 (H4) cells. Interestingly, we observed an increase in the levels of Bax, cleavage of PARP-1, and mitochondrial release of proapoptotic proteins, such as cytochrome c and HtrA2, in H4 cells with intracellular Aβ or C99 aggregates, but not in HEK293 cells with those aggregates. The results of the present study indicate that intracellular Aβ and C99 aggregates induce mitochondria-dependent apoptotic cell death via elevation of Bax levels as a result of proteasome inhibition in a cell type-specific manner.
KW - Alzheimer's disease
KW - Amyloid beta (Aβ)
KW - C99
KW - Human neuroglioma H4 cell
KW - Mitochondria-dependent cell death
KW - Proteasome
UR - http://www.scopus.com/inward/record.url?scp=67349132288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349132288&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2009.04.001
DO - 10.1016/j.brainres.2009.04.001
M3 - Article
C2 - 19362074
AN - SCOPUS:67349132288
VL - 1273
SP - 1
EP - 8
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -