TY - GEN
T1 - Intracellular delivery of active biomolecules through vortex-induced cell deformation
AU - Hur, Jeongsoo
AU - Chung, Aram J.
N1 - Funding Information:
The work is partially supported by Korea University Grant, and National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A1B07045538).
PY - 2019
Y1 - 2019
N2 - We present a novel vortex-based microfluidic intracellular delivery platform that can deliver large biomolecules into cells in a single step. This approach utilizes intrinsic vortices developed in a T-junction microchannel with a micro-cavity to hydrodynamically open cellular membranes, delivering external biomolecules into cytoplasm or nucleus. In brief, the cell suspension mixed with target materials is injected into the microchannel where cells undergo vortex-induced cell deformation. These hydrodynamic cell disruptions create transient nanopores allowing introduction of macro-biomolecules. Using this process, we achieved highly efficient intracellular delivery (<98%) in a high-throughput manner (~1,000,000 cells/min) while maintaining high cell viability (<95%).
AB - We present a novel vortex-based microfluidic intracellular delivery platform that can deliver large biomolecules into cells in a single step. This approach utilizes intrinsic vortices developed in a T-junction microchannel with a micro-cavity to hydrodynamically open cellular membranes, delivering external biomolecules into cytoplasm or nucleus. In brief, the cell suspension mixed with target materials is injected into the microchannel where cells undergo vortex-induced cell deformation. These hydrodynamic cell disruptions create transient nanopores allowing introduction of macro-biomolecules. Using this process, we achieved highly efficient intracellular delivery (<98%) in a high-throughput manner (~1,000,000 cells/min) while maintaining high cell viability (<95%).
KW - Cell Transfection
KW - Gene Delivery
KW - Hydroporation
KW - Hydroporator
KW - Intracellular delivery
UR - http://www.scopus.com/inward/record.url?scp=85094974038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094974038&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094974038
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 176
EP - 177
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -