@inproceedings{dc0f31575fe446388d4539f3f7b64ab0,
title = "Intracellular delivery of active biomolecules through vortex-induced cell deformation",
abstract = "We present a novel vortex-based microfluidic intracellular delivery platform that can deliver large biomolecules into cells in a single step. This approach utilizes intrinsic vortices developed in a T-junction microchannel with a micro-cavity to hydrodynamically open cellular membranes, delivering external biomolecules into cytoplasm or nucleus. In brief, the cell suspension mixed with target materials is injected into the microchannel where cells undergo vortex-induced cell deformation. These hydrodynamic cell disruptions create transient nanopores allowing introduction of macro-biomolecules. Using this process, we achieved highly efficient intracellular delivery (<98%) in a high-throughput manner (~1,000,000 cells/min) while maintaining high cell viability (<95%).",
keywords = "Cell Transfection, Gene Delivery, Hydroporation, Hydroporator, Intracellular delivery",
author = "Jeongsoo Hur and Chung, {Aram J.}",
note = "Funding Information: The work is partially supported by Korea University Grant, and National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A1B07045538). Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",
year = "2019",
language = "English",
series = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",
publisher = "Chemical and Biological Microsystems Society",
pages = "176--177",
booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",
}