Intracellular delivery of active biomolecules through vortex-induced cell deformation

Jeongsoo Hur, Aram J. Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel vortex-based microfluidic intracellular delivery platform that can deliver large biomolecules into cells in a single step. This approach utilizes intrinsic vortices developed in a T-junction microchannel with a micro-cavity to hydrodynamically open cellular membranes, delivering external biomolecules into cytoplasm or nucleus. In brief, the cell suspension mixed with target materials is injected into the microchannel where cells undergo vortex-induced cell deformation. These hydrodynamic cell disruptions create transient nanopores allowing introduction of macro-biomolecules. Using this process, we achieved highly efficient intracellular delivery (<98%) in a high-throughput manner (~1,000,000 cells/min) while maintaining high cell viability (<95%).

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages176-177
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Cell Transfection
  • Gene Delivery
  • Hydroporation
  • Hydroporator
  • Intracellular delivery

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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