TY - JOUR
T1 - Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide
AU - Kim, Jungyeon
AU - Kim, Joongsuk
AU - Um, Youngsoon
AU - Kim, Kyoung Heon
N1 - Funding Information:
We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.
Funding Information:
We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea ( 2016M3D3A1A01913268 ). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Appendix A
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/2
Y1 - 2020/2
N2 - Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.
AB - Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.
KW - Carbon monoxide
KW - Clostridium carboxidivorans
KW - Extraction solvent
KW - Metabolomics
KW - Optimization
UR - http://www.scopus.com/inward/record.url?scp=85075423815&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2019.10.012
DO - 10.1016/j.procbio.2019.10.012
M3 - Article
AN - SCOPUS:85075423815
SN - 1359-5113
VL - 89
SP - 20
EP - 28
JO - Process Biochemistry
JF - Process Biochemistry
ER -