Abstract
Previous studies have demonstrated that the epithelium of the respiratory portion of rat nasal mucosa is amply supplied by nerve fibers with immuno-reactivities for calcitonin gene-related peptide (CGRP) and substance P (SP), these fibers most likely acting as sensory mediators in the mucosa. The present study demonstrates that some intraepithelial fibers contain a VIP-immunoreactivity whose occurrence in these nerves has previously been neglected. The present study further aims to confirm the occurrence of NO-producing intraepithelial nerve fibers in the rat nasal mucosa and to examine its colocalization with CGRP and with VIP. Double staining methods were used to evaluate the colocalization of NADPH-diaphorase reactivity and CGRP- or VIP-immunoreactivity. The reactivity for NADPH-diaphorase and that for CGRP coexisted in only a small part, if any, of the nerve fibers distributed at the basal portion of the epithelium. In the perpendicularly and obliquely oriented transepithelial nerve fibers, both reactivities were clearly demonstrated to be separated in different fibers. VIP immunoreactivity was also present in a part of the intraepithelial nerve fibers of the nasal mucosa, and their entire population was shown to be positive for NADPH-diaphorase. The NADPH-diaphorase-positive reaction was displayed in only a small population of neurons in the trigeminal ganglion, whereas it was seen in numerous neurons in sphenopalatine ganglion, being colocalized with VIP.
| Original language | English |
|---|---|
| Pages (from-to) | 449-456 |
| Number of pages | 8 |
| Journal | Archives of Histology and Cytology |
| Volume | 58 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1995 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Histology
Cite this
Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO). / Hag Sang, Lee; Iwanaga, Toshihiko; Fujita, Tsuneo; Lee, Sang Hag.
In: Archives of Histology and Cytology, Vol. 58, No. 4, 01.01.1995, p. 449-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intraepithelial Nerve Fibers in the Nasal Mucosa of the Rat with Special Reference to the Localization of CGRP, VIP and Nitric Oxide (NO)
AU - Hag Sang, Lee
AU - Iwanaga, Toshihiko
AU - Fujita, Tsuneo
AU - Lee, Sang Hag
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Previous studies have demonstrated that the epithelium of the respiratory portion of rat nasal mucosa is amply supplied by nerve fibers with immuno-reactivities for calcitonin gene-related peptide (CGRP) and substance P (SP), these fibers most likely acting as sensory mediators in the mucosa. The present study demonstrates that some intraepithelial fibers contain a VIP-immunoreactivity whose occurrence in these nerves has previously been neglected. The present study further aims to confirm the occurrence of NO-producing intraepithelial nerve fibers in the rat nasal mucosa and to examine its colocalization with CGRP and with VIP. Double staining methods were used to evaluate the colocalization of NADPH-diaphorase reactivity and CGRP- or VIP-immunoreactivity. The reactivity for NADPH-diaphorase and that for CGRP coexisted in only a small part, if any, of the nerve fibers distributed at the basal portion of the epithelium. In the perpendicularly and obliquely oriented transepithelial nerve fibers, both reactivities were clearly demonstrated to be separated in different fibers. VIP immunoreactivity was also present in a part of the intraepithelial nerve fibers of the nasal mucosa, and their entire population was shown to be positive for NADPH-diaphorase. The NADPH-diaphorase-positive reaction was displayed in only a small population of neurons in the trigeminal ganglion, whereas it was seen in numerous neurons in sphenopalatine ganglion, being colocalized with VIP.
AB - Previous studies have demonstrated that the epithelium of the respiratory portion of rat nasal mucosa is amply supplied by nerve fibers with immuno-reactivities for calcitonin gene-related peptide (CGRP) and substance P (SP), these fibers most likely acting as sensory mediators in the mucosa. The present study demonstrates that some intraepithelial fibers contain a VIP-immunoreactivity whose occurrence in these nerves has previously been neglected. The present study further aims to confirm the occurrence of NO-producing intraepithelial nerve fibers in the rat nasal mucosa and to examine its colocalization with CGRP and with VIP. Double staining methods were used to evaluate the colocalization of NADPH-diaphorase reactivity and CGRP- or VIP-immunoreactivity. The reactivity for NADPH-diaphorase and that for CGRP coexisted in only a small part, if any, of the nerve fibers distributed at the basal portion of the epithelium. In the perpendicularly and obliquely oriented transepithelial nerve fibers, both reactivities were clearly demonstrated to be separated in different fibers. VIP immunoreactivity was also present in a part of the intraepithelial nerve fibers of the nasal mucosa, and their entire population was shown to be positive for NADPH-diaphorase. The NADPH-diaphorase-positive reaction was displayed in only a small population of neurons in the trigeminal ganglion, whereas it was seen in numerous neurons in sphenopalatine ganglion, being colocalized with VIP.
UR - http://www.scopus.com/inward/record.url?scp=0028793930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028793930&partnerID=8YFLogxK
U2 - 10.1679/aohc.58.449
DO - 10.1679/aohc.58.449
M3 - Article
C2 - 8562135
AN - SCOPUS:0028793930
VL - 58
SP - 449
EP - 456
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 4
ER -