Abstract
Bare gold nanospheres have been shown to have anti-angiogenic effects but are optically unfavorable because their resonant wavelength lies in the visible spectrum. Here, we design gold nanodisks with a higher scattering capability than gold nanorods and with a resonant wavelength at near-infrared region – the area where the source of light utilized by optical coherence tomography (OCT) lies. With a physical synthesis system, we then fabricate 160-nm-sized gold nanodisks exhibiting resonant wavelength at 830 nm. The synthesized nanoparticles were successfully visualized in in vivo OCT at concentrations as low as 1 pM. After demonstrating their binding ability to vascular endothelial growth factor (VEGF), we show that they suppress VEGF-induced migration of endothelial cells. Finally, we demonstrate that intravitreally injected gold nanodisks attenuate neovascularization of oxygen-induced retinopathy in mice, in a dose dependent manner, such that they are cleared from the vitreous within 2 weeks without histologic or electrophysiologic toxicity.
Original language | English |
---|---|
Pages (from-to) | 1901-1911 |
Number of pages | 11 |
Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
Volume | 13 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2017 Aug 1 |
Externally published | Yes |
Keywords
- Angiogenesis inhibitor
- Gold nanodisk
- Intraocular application
- Near-infrared
- Optical coherence tomography
- Retinal neovascularization
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Bioengineering
- Biomedical Engineering
- Molecular Medicine
- Materials Science(all)
- Pharmaceutical Science