Abstract
Bare gold nanospheres have been shown to have anti-angiogenic effects but are optically unfavorable because their resonant wavelength lies in the visible spectrum. Here, we design gold nanodisks with a higher scattering capability than gold nanorods and with a resonant wavelength at near-infrared region – the area where the source of light utilized by optical coherence tomography (OCT) lies. With a physical synthesis system, we then fabricate 160-nm-sized gold nanodisks exhibiting resonant wavelength at 830 nm. The synthesized nanoparticles were successfully visualized in in vivo OCT at concentrations as low as 1 pM. After demonstrating their binding ability to vascular endothelial growth factor (VEGF), we show that they suppress VEGF-induced migration of endothelial cells. Finally, we demonstrate that intravitreally injected gold nanodisks attenuate neovascularization of oxygen-induced retinopathy in mice, in a dose dependent manner, such that they are cleared from the vitreous within 2 weeks without histologic or electrophysiologic toxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 1901-1911 |
| Number of pages | 11 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 13 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2017 Aug 1 |
| Externally published | Yes |
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Keywords
- Angiogenesis inhibitor
- Gold nanodisk
- Intraocular application
- Near-infrared
- Optical coherence tomography
- Retinal neovascularization
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Bioengineering
- Biomedical Engineering
- Molecular Medicine
- Materials Science(all)
- Pharmaceutical Science
Cite this
Intraocular application of gold nanodisks optically tuned for optical coherence tomography : inhibitory effect on retinal neovascularization without unbearable toxicity. / Song, Hyun Beom; Wi, Jung Sub; Jo, Dong Hyun; Kim, Jin Hyoung; Lee, Sang-Won; Lee, Tae Geol; Kim, Jeong Hun.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 13, No. 6, 01.08.2017, p. 1901-1911.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intraocular application of gold nanodisks optically tuned for optical coherence tomography
T2 - inhibitory effect on retinal neovascularization without unbearable toxicity
AU - Song, Hyun Beom
AU - Wi, Jung Sub
AU - Jo, Dong Hyun
AU - Kim, Jin Hyoung
AU - Lee, Sang-Won
AU - Lee, Tae Geol
AU - Kim, Jeong Hun
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Bare gold nanospheres have been shown to have anti-angiogenic effects but are optically unfavorable because their resonant wavelength lies in the visible spectrum. Here, we design gold nanodisks with a higher scattering capability than gold nanorods and with a resonant wavelength at near-infrared region – the area where the source of light utilized by optical coherence tomography (OCT) lies. With a physical synthesis system, we then fabricate 160-nm-sized gold nanodisks exhibiting resonant wavelength at 830 nm. The synthesized nanoparticles were successfully visualized in in vivo OCT at concentrations as low as 1 pM. After demonstrating their binding ability to vascular endothelial growth factor (VEGF), we show that they suppress VEGF-induced migration of endothelial cells. Finally, we demonstrate that intravitreally injected gold nanodisks attenuate neovascularization of oxygen-induced retinopathy in mice, in a dose dependent manner, such that they are cleared from the vitreous within 2 weeks without histologic or electrophysiologic toxicity.
AB - Bare gold nanospheres have been shown to have anti-angiogenic effects but are optically unfavorable because their resonant wavelength lies in the visible spectrum. Here, we design gold nanodisks with a higher scattering capability than gold nanorods and with a resonant wavelength at near-infrared region – the area where the source of light utilized by optical coherence tomography (OCT) lies. With a physical synthesis system, we then fabricate 160-nm-sized gold nanodisks exhibiting resonant wavelength at 830 nm. The synthesized nanoparticles were successfully visualized in in vivo OCT at concentrations as low as 1 pM. After demonstrating their binding ability to vascular endothelial growth factor (VEGF), we show that they suppress VEGF-induced migration of endothelial cells. Finally, we demonstrate that intravitreally injected gold nanodisks attenuate neovascularization of oxygen-induced retinopathy in mice, in a dose dependent manner, such that they are cleared from the vitreous within 2 weeks without histologic or electrophysiologic toxicity.
KW - Angiogenesis inhibitor
KW - Gold nanodisk
KW - Intraocular application
KW - Near-infrared
KW - Optical coherence tomography
KW - Retinal neovascularization
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UR - http://www.scopus.com/inward/citedby.url?scp=85020818730&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2017.03.016
DO - 10.1016/j.nano.2017.03.016
M3 - Article
C2 - 28400160
AN - SCOPUS:85020818730
VL - 13
SP - 1901
EP - 1911
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 6
ER -