Intraoperative combined color and fluorescent images-based sentinel node mapping in the porcine lung: Comparison of indocyanine green with or without albumin premixing

Yujin Oh, Yu Hua Quan, Yeonho Choi, Choong Ki Kim, Heezoo Kim, Hyun Koo Kim, Beop-Min Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)


Objectives: We developed a multimodal optical imaging system for intraoperative visualization of sentinel lymph nodes (SLNs). This study is to validate our system by showing SLNs in the lung through combined optical color and fluorescent image with indocyanine green (ICG) and ICG with human serum albumin (HSA). Methods: Identical ICG concentrations of ICG only or ICG:HSA was injected into the rat footpad and porcine lung. Absolute amounts of the fluorescents were scaled on the basis of animal weights. The entire procedures were recorded using color and near-infrared (NIR) charge-coupled device (CCD) cameras simultaneously, and the 2 images were merged by real-time image processing software. All fluorescence intensity signals to background ratio (SBR) and retention rates at SLN for both fluorescents were estimated and compared. Results: This newly developed intraoperative color and fluorescence optical imaging system successfully visualized the SLNs in animal experiments. The SLNs were identified 100% for both rat and pig under in vivo conditions. Real-time image processing software overcame the low signal of NIR fluorescence images. ICG and ICG:HSA provided no significantly different SBR in the SLN images for both rat thigh and pig lung. Conclusions: The intraoperative optical imaging system enabled real-time image-guided surgery during SLN mapping in lung in an animal model. The ICG retention rate was similar to ICG:HSA. ICG alone can be useful for SLN imaging during lung cancer surgery.

Original languageEnglish
Pages (from-to)1509-1515
Number of pages7
JournalJournal of Thoracic and Cardiovascular Surgery
Issue number6
Publication statusPublished - 2013 Dec 1


ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

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