Intraparticle energy transfer and fluorescence photoconversion in nanoparticles: An optical highlighter nanoprobe for two-photon bioimaging

Sehoon Kim, Heng Huang, Haridas E. Pudavar, Yiping Cui, Paras N. Prasad

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We report organically modified silica nanoparticles co-encapsulating two-photon fluorescent dye aggregates and a red-fluorescent energy acceptor, where the latter is indirectly excited through intraparticle energy transfer following two-photon excitation of the former. In vitro two-photon microscopy has demonstrated that these nanoprobes are avidly internalized into cells with bright acceptor fluorescence and that cellular optical highlighting is effected with red-to-green photoconversion by selective acceptor bleaching, while keeping the donor and the nanostructure of particles intact.

Original languageEnglish
Pages (from-to)5650-5656
Number of pages7
JournalChemistry of Materials
Volume19
Issue number23
DOIs
Publication statusPublished - 2007 Nov 13
Externally publishedYes

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Nanoprobes
Energy transfer
Photons
Fluorescence
Nanoparticles
Bleaching
Fluorescent Dyes
Silicon Dioxide
Nanostructures
Microscopic examination
Dyes
Silica

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Intraparticle energy transfer and fluorescence photoconversion in nanoparticles : An optical highlighter nanoprobe for two-photon bioimaging. / Kim, Sehoon; Huang, Heng; Pudavar, Haridas E.; Cui, Yiping; Prasad, Paras N.

In: Chemistry of Materials, Vol. 19, No. 23, 13.11.2007, p. 5650-5656.

Research output: Contribution to journalArticle

Kim, Sehoon ; Huang, Heng ; Pudavar, Haridas E. ; Cui, Yiping ; Prasad, Paras N. / Intraparticle energy transfer and fluorescence photoconversion in nanoparticles : An optical highlighter nanoprobe for two-photon bioimaging. In: Chemistry of Materials. 2007 ; Vol. 19, No. 23. pp. 5650-5656.
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