Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells

Yingyu Qin, Sejin Oh, Sojung Lim, Jung Hoon Shin, Min Sang Yoon, Se Ho Park

Research output: Contribution to journalArticle

Abstract

Invariant natural killer T (iNKT) cells are a major subset of NKT cells that recognize foreign and endogenous lipid antigens presented by CD1d. Although iNKT cells are characteristically autoreactive to self-antigens, the role of iNKT cells in the regulation of cytotoxic T lymphocytes (CTL) has been elucidated using α-galactosylceramide (α-GalCer), a strong synthetic glycolipid that is presented by professional antigen presenting cells (APCs), such as dendritic cells. Despite the well-known effects of α-GalCer and dendritic cells on lipid antigen presentation, the physiological role of endogenous antigens presented by CTLs during crosstalk with iNKT cells has not yet been addressed. In this study, we found that antigen-primed CTLs with transient CD1d upregulation could present lipid self-antigens to activate the iNKT cell production of IFN-γ. CTL-mediated iNKT cell activation in turn enhanced IFN-γ production and the proliferation and cytotoxicity of CTLs. We also found that the direct interaction of iNKT cells and CTLs enhanced the antitumor immune responses of CTLs. This partially explains the functional role of iNKT cells in CTL-mediated antitumor immunity. Our findings suggest that in the absence of exogenous iNKT cell ligands, iNKT cells enhanced the CTL production of IFN-γ and CTL proliferation and cytotoxicity via direct interaction with CD1d expressed on T cells without interacting with APCs.

Original languageEnglish
Article number126
JournalExperimental and Molecular Medicine
Volume51
Issue number10
DOIs
Publication statusPublished - 2019 Oct 1

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Natural Killer T-Cells
T-cells
Chemical activation
T-Lymphocytes
Cytotoxic T-Lymphocytes
Autoantigens
Antigen-Presenting Cells
Lipids
Dendritic Cells
CD1d Antigen
Cytotoxicity
Galactosylceramides
Antigens
Glycolipids
Antigen Presentation
Immunity
Up-Regulation
Crosstalk
Ligands

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells. / Qin, Yingyu; Oh, Sejin; Lim, Sojung; Shin, Jung Hoon; Yoon, Min Sang; Park, Se Ho.

In: Experimental and Molecular Medicine, Vol. 51, No. 10, 126, 01.10.2019.

Research output: Contribution to journalArticle

Qin, Yingyu ; Oh, Sejin ; Lim, Sojung ; Shin, Jung Hoon ; Yoon, Min Sang ; Park, Se Ho. / Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells. In: Experimental and Molecular Medicine. 2019 ; Vol. 51, No. 10.
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