Inverse modulation of the energy sensor Snf1-related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana

Jong Hee Im, Young Hee Cho, Geun Don Kim, Geun Ho Kang, Jung Woo Hong, Sang-Dong Yoo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Terrestrial plants are exposed to complex stresses of high salt-induced abscisic acid (ABA) and submergence-induced hypoxia when seawater floods fields. Many studies have investigated plant responses to individual stress conditions, but not so much for coupled or sequentially imposed stresses. We examined molecular regulatory mechanisms of gene expression underlying the cellular responses involved in crosstalk between salt and hypoxia stresses. Salt/ABA- and AtMYC2-dependent induction of a synthetic ABA-responsive element and the native RD22 promoters were utilized in our cell-based functional assays. Such promoter-based reporter induction was largely inhibited by hypoxia and hypoxia-inducible AKIN10 activity. Biochemical analyses showed that AKIN10 negatively modulates AtMYC2 protein accumulation via proteasome activity upon AKIN10 kinase activity-dependent protein modification. Further genetic analysis using transgenic plants expressing AKIN10 provided evidence that AKIN10 activity undermined AtMYC2-dependent salt tolerance. Our findings unravel a novel molecular interaction between the key signalling constituents leading crosstalk between salt and hypoxia stresses in Arabidopsis thaliana under the detrimental condition of submergence in saltwater.

Original languageEnglish
Pages (from-to)2303-2312
Number of pages10
JournalPlant, Cell and Environment
Volume37
Issue number10
DOIs
Publication statusPublished - 2014 Jan 1

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Salt-Tolerance
Arabidopsis
protein kinases
stress tolerance
Protein Kinases
salt stress
hypoxia
Arabidopsis thaliana
Abscisic Acid
Salts
energy
salts
abscisic acid
submergence
promoter regions
Genetically Modified Plants
proteasome endopeptidase complex
Seawater
Proteasome Endopeptidase Complex
Regulator Genes

Cite this

Inverse modulation of the energy sensor Snf1-related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana. / Im, Jong Hee; Cho, Young Hee; Kim, Geun Don; Kang, Geun Ho; Hong, Jung Woo; Yoo, Sang-Dong.

In: Plant, Cell and Environment, Vol. 37, No. 10, 01.01.2014, p. 2303-2312.

Research output: Contribution to journalArticle

Im, Jong Hee ; Cho, Young Hee ; Kim, Geun Don ; Kang, Geun Ho ; Hong, Jung Woo ; Yoo, Sang-Dong. / Inverse modulation of the energy sensor Snf1-related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana. In: Plant, Cell and Environment. 2014 ; Vol. 37, No. 10. pp. 2303-2312.
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