Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system

Deok Ho Kim; Yu Sun; Seok Yun; Ho Lee Sang; Byungkyu Kim

doi:10.1016/j.jbiomech.2004.07.014

Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system

Deok Ho Kim, Yu Sun, Seok Yun, Ho Lee Sang, Byungkyu Kim

* Honorary professors

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The zebrafish is a model organism for addressing questions of vertebrate embryo development. In this paper, the softening phenomenon of the chorion envelope of zebrafish embryos at different developmental stages was mechanically quantitated by using a microrobotic force sensing system. The microrobotic system integrates a piezoelectric cellular force sensor to measure the required forces for penetrating the chorion envelope. Magnitude of penetration forces was found to decrease as an embryo develops. The results mechanically quantitate "chorion softening" in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development.

Original language	English
Pages (from-to)	1359-1363
Number of pages	5
Journal	Journal of Biomechanics
Volume	38
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiomech.2004.07.014
Publication status	Published - 2005 Jun

Keywords

Chorion envelope
Chorion softening
Force sensing
Microrobotic system
Protease
Zebrafish embryo

ASJC Scopus subject areas

Biophysics
Orthopedics and Sports Medicine
Biomedical Engineering
Rehabilitation

Access to Document

10.1016/j.jbiomech.2004.07.014

Cite this

@article{cd28b62692724d9d9439b81172530a48,

title = "Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system",

abstract = "The zebrafish is a model organism for addressing questions of vertebrate embryo development. In this paper, the softening phenomenon of the chorion envelope of zebrafish embryos at different developmental stages was mechanically quantitated by using a microrobotic force sensing system. The microrobotic system integrates a piezoelectric cellular force sensor to measure the required forces for penetrating the chorion envelope. Magnitude of penetration forces was found to decrease as an embryo develops. The results mechanically quantitate {"}chorion softening{"} in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development.",

keywords = "Chorion envelope, Chorion softening, Force sensing, Microrobotic system, Protease, Zebrafish embryo",

author = "Kim, {Deok Ho} and Yu Sun and Seok Yun and Sang, {Ho Lee} and Byungkyu Kim",

note = "Funding Information: This work was supported by the 21st Century's Frontier R&D Projects, under the Contract no. MS-02-324-01, sponsored by the Ministry of Science and Technology, Korea. We thank C. Hwang for special contribution in obtaining the zebrafish embryos and H. Kang for the collaboration in developing the early experimental setup.",

year = "2005",

month = jun,

doi = "10.1016/j.jbiomech.2004.07.014",

language = "English",

volume = "38",

pages = "1359--1363",

journal = "Journal of Biomechanics",

issn = "0021-9290",

publisher = "Elsevier Limited",

number = "6",

}

TY - JOUR

T1 - Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system

AU - Kim, Deok Ho

AU - Sun, Yu

AU - Yun, Seok

AU - Sang, Ho Lee

AU - Kim, Byungkyu

N1 - Funding Information: This work was supported by the 21st Century's Frontier R&D Projects, under the Contract no. MS-02-324-01, sponsored by the Ministry of Science and Technology, Korea. We thank C. Hwang for special contribution in obtaining the zebrafish embryos and H. Kang for the collaboration in developing the early experimental setup.

PY - 2005/6

Y1 - 2005/6

N2 - The zebrafish is a model organism for addressing questions of vertebrate embryo development. In this paper, the softening phenomenon of the chorion envelope of zebrafish embryos at different developmental stages was mechanically quantitated by using a microrobotic force sensing system. The microrobotic system integrates a piezoelectric cellular force sensor to measure the required forces for penetrating the chorion envelope. Magnitude of penetration forces was found to decrease as an embryo develops. The results mechanically quantitate "chorion softening" in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development.

AB - The zebrafish is a model organism for addressing questions of vertebrate embryo development. In this paper, the softening phenomenon of the chorion envelope of zebrafish embryos at different developmental stages was mechanically quantitated by using a microrobotic force sensing system. The microrobotic system integrates a piezoelectric cellular force sensor to measure the required forces for penetrating the chorion envelope. Magnitude of penetration forces was found to decrease as an embryo develops. The results mechanically quantitate "chorion softening" in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development.

KW - Chorion envelope

KW - Chorion softening

KW - Force sensing

KW - Microrobotic system

KW - Protease

KW - Zebrafish embryo

UR - http://www.scopus.com/inward/record.url?scp=18044389890&partnerID=8YFLogxK

U2 - 10.1016/j.jbiomech.2004.07.014

DO - 10.1016/j.jbiomech.2004.07.014

M3 - Article

C2 - 15940808

AN - SCOPUS:18044389890

SN - 0021-9290

VL - 38

SP - 1359

EP - 1363

JO - Journal of Biomechanics

JF - Journal of Biomechanics

IS - 6

ER -

Investigating chorion softening of zebrafish embryos with a microrobotic force sensing system

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this