Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells

Eunyi Jeon, Ye Rang Yun, Wonmo Kang, Sujin Lee, Young-Hag Koh, Hae Won Kim, Chang Kook Suh, Jun Hyeog Jang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (plt;0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.

Original languageEnglish
Article numbere43982
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - 2012 Aug 24

Fingerprint

Fibroblasts
Stem cells
Mesenchymal Stromal Cells
fibroblasts
stem cells
Growth
Rats
Bone
rats
bone marrow cells
mineralization
Bone Marrow
bones
Bone Morphogenetic Protein 4
Hematopoietic System
bone morphogenetic proteins
Bone Regeneration
Collagen Type I
collagen
Assays

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells. / Jeon, Eunyi; Yun, Ye Rang; Kang, Wonmo; Lee, Sujin; Koh, Young-Hag; Kim, Hae Won; Suh, Chang Kook; Jang, Jun Hyeog.

In: PLoS One, Vol. 7, No. 8, e43982, 24.08.2012.

Research output: Contribution to journalArticle

Jeon, Eunyi ; Yun, Ye Rang ; Kang, Wonmo ; Lee, Sujin ; Koh, Young-Hag ; Kim, Hae Won ; Suh, Chang Kook ; Jang, Jun Hyeog. / Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells. In: PLoS One. 2012 ; Vol. 7, No. 8.
@article{e13ad6664aec4c1e82f4373b782f2e5b,
title = "Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells",
abstract = "Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (plt;0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.",
author = "Eunyi Jeon and Yun, {Ye Rang} and Wonmo Kang and Sujin Lee and Young-Hag Koh and Kim, {Hae Won} and Suh, {Chang Kook} and Jang, {Jun Hyeog}",
year = "2012",
month = "8",
day = "24",
doi = "10.1371/journal.pone.0043982",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells

AU - Jeon, Eunyi

AU - Yun, Ye Rang

AU - Kang, Wonmo

AU - Lee, Sujin

AU - Koh, Young-Hag

AU - Kim, Hae Won

AU - Suh, Chang Kook

AU - Jang, Jun Hyeog

PY - 2012/8/24

Y1 - 2012/8/24

N2 - Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (plt;0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.

AB - Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (plt;0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.

UR - http://www.scopus.com/inward/record.url?scp=84866607725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866607725&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0043982

DO - 10.1371/journal.pone.0043982

M3 - Article

C2 - 22937141

AN - SCOPUS:84866607725

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e43982

ER -