Investigation of β-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea

Eui Suk Sohn, Jeong Sik Yoo, Jeom Kyu Lee, Kyeong Min Lee, Gyung Tae Chung, Eun Shim Shin, Sun Young Han, Sang Hee Lee, Joon Kim, Yeong Seon Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of β-lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the blaOXA-10, blaOXA-2, blaOXA-17, blaPER-1, blaSHV-12, and blaIMP-1 genes were carried by 34.3%, 26.9%, 3.0%, 3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of β-lactamase genes were carried by 10.4% of isolates. The most prevalent β-lactamase genes were blaVIM-2 and blaOXA-10. This study is the first description of MDR P. aeruginosa from non-tertiary care hospitals in Korea and the coexistence of the blaOXA-10 gene with blaVIM-2, blaIMP-1, or blaPER-1, in these clinical isolates.

Original languageEnglish
Pages (from-to)1733-1737
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number10
Publication statusPublished - 2007 Oct 1

Fingerprint

Korea
Pseudomonas aeruginosa
Genes
Monobactams
Carbapenems
Quinolones
Ethylenediaminetetraacetic acid
Aminoglycosides
Cephalosporins
Polymerase Chain Reaction
Edetic Acid
Penicillins
Amplification
Microbial Sensitivity Tests

Keywords

  • Multidrug resistance
  • OXA-10
  • PER-1
  • Pseudomonas aeruginosa
  • VIM-2

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Sohn, E. S., Yoo, J. S., Lee, J. K., Lee, K. M., Chung, G. T., Shin, E. S., ... Lee, Y. S. (2007). Investigation of β-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea. Journal of Microbiology and Biotechnology, 17(10), 1733-1737.

Investigation of β-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea. / Sohn, Eui Suk; Yoo, Jeong Sik; Lee, Jeom Kyu; Lee, Kyeong Min; Chung, Gyung Tae; Shin, Eun Shim; Han, Sun Young; Lee, Sang Hee; Kim, Joon; Lee, Yeong Seon.

In: Journal of Microbiology and Biotechnology, Vol. 17, No. 10, 01.10.2007, p. 1733-1737.

Research output: Contribution to journalArticle

Sohn, ES, Yoo, JS, Lee, JK, Lee, KM, Chung, GT, Shin, ES, Han, SY, Lee, SH, Kim, J & Lee, YS 2007, 'Investigation of β-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea', Journal of Microbiology and Biotechnology, vol. 17, no. 10, pp. 1733-1737.
Sohn, Eui Suk ; Yoo, Jeong Sik ; Lee, Jeom Kyu ; Lee, Kyeong Min ; Chung, Gyung Tae ; Shin, Eun Shim ; Han, Sun Young ; Lee, Sang Hee ; Kim, Joon ; Lee, Yeong Seon. / Investigation of β-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea. In: Journal of Microbiology and Biotechnology. 2007 ; Vol. 17, No. 10. pp. 1733-1737.
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