Investigation of active anti-inflammatory constituents of essential oil from Pinus koraiensis (Sieb. et zucc.) wood in LPS-stimulated RBL-2H3 cells

Ji Yoon Yang, Won Sil Choi, Ki Joong Kim, Chang Deuk Eom, Mi Jin Park

Research output: Contribution to journalArticlepeer-review

Abstract

In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (Pinus koraiensis, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of P. koraiensis oil. The essential oil was extracted from P. koraiensis wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of β-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-α-pinene, (−)-β-pinene, (+)-α-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of βhexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-α-Pinene, (−)-β-pinene, (+)-α-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.

Original languageEnglish
Article number817
JournalBiomolecules
Volume11
Issue number6
DOIs
Publication statusPublished - 2021 Jun

Keywords

  • Active compound
  • Anti-inflammatory
  • Essential oil
  • Longifolene
  • Pinus koraiensis
  • α-pinene
  • α-terpineol
  • β-pinene

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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