Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park, Deok Hie Park, Soohwan Sul, Sunghwan F. Oh, In Sook Park, Doo Soo Chung, Hie Joon Kim, Min Sik Kim, Sang-Won Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

Original languageEnglish
Pages (from-to)1791-1800
Number of pages10
JournalBulletin of the Korean Chemical Society
Volume25
Issue number12
Publication statusPublished - 2004 Dec 20

Fingerprint

Glycosylation
Electrospray ionization
Angiotensins
Ionization
Mass spectrometry
Desorption
Cathepsin A
Angiotensin I
Peptides
Lasers
Guanidine
Aspartic Acid
Arginine
Acids
lysyl aminopeptidase

Keywords

  • Angiotensin
  • ESI MS/MS
  • Glycosylation
  • MALDI-TOF MS
  • Partial hydrolysis

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Park, S. J., Park, D. H., Sul, S., Oh, S. F., Park, I. S., Chung, D. S., ... Lee, S-W. (2004). Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry. Bulletin of the Korean Chemical Society, 25(12), 1791-1800.

Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry. / Park, Soo Jin; Park, Deok Hie; Sul, Soohwan; Oh, Sunghwan F.; Park, In Sook; Chung, Doo Soo; Kim, Hie Joon; Kim, Min Sik; Lee, Sang-Won.

In: Bulletin of the Korean Chemical Society, Vol. 25, No. 12, 20.12.2004, p. 1791-1800.

Research output: Contribution to journalArticle

Park, SJ, Park, DH, Sul, S, Oh, SF, Park, IS, Chung, DS, Kim, HJ, Kim, MS & Lee, S-W 2004, 'Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry', Bulletin of the Korean Chemical Society, vol. 25, no. 12, pp. 1791-1800.
Park SJ, Park DH, Sul S, Oh SF, Park IS, Chung DS et al. Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry. Bulletin of the Korean Chemical Society. 2004 Dec 20;25(12):1791-1800.
Park, Soo Jin ; Park, Deok Hie ; Sul, Soohwan ; Oh, Sunghwan F. ; Park, In Sook ; Chung, Doo Soo ; Kim, Hie Joon ; Kim, Min Sik ; Lee, Sang-Won. / Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry. In: Bulletin of the Korean Chemical Society. 2004 ; Vol. 25, No. 12. pp. 1791-1800.
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AU - Chung, Doo Soo

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