Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park, Deok Hie Park, Soohwan Sul, Sunghwan F. Oh, In Sook Park, Doo Soo Chung, Hie Joon Kim, Min Sik Kim, Sang Won Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

Original languageEnglish
Pages (from-to)1791-1800
Number of pages10
JournalBulletin of the Korean Chemical Society
Volume25
Issue number12
DOIs
Publication statusPublished - 2004 Dec 20

Keywords

  • Angiotensin
  • ESI MS/MS
  • Glycosylation
  • MALDI-TOF MS
  • Partial hydrolysis

ASJC Scopus subject areas

  • Chemistry(all)

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  • Cite this

    Park, S. J., Park, D. H., Sul, S., Oh, S. F., Park, I. S., Chung, D. S., Kim, H. J., Kim, M. S., & Lee, S. W. (2004). Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry. Bulletin of the Korean Chemical Society, 25(12), 1791-1800. https://doi.org/10.5012/bkcs.2004.25.12.1791