Abstract
Purpose: Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation. Methods: We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach. Results: Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue. Conclusions: Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
| Original language | English |
|---|---|
| Pages (from-to) | 255-262 |
| Number of pages | 8 |
| Journal | Journal of Cancer Research and Clinical Oncology |
| Volume | 134 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Feb 1 |
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Keywords
- Malignant transformation
- Mass spectrometry
- Oligodendroglioma
- Peroxiredoxin
- Rho GDP dissociation inhibitor
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Investigation of molecular factors associated with malignant transformation of oligodendroglioma by proteomic study of a single case of rapid tumor progression. / Park, Chul Kee; Kim, Jin Hyun; Moon, Min Jeong; Jung, Ji Hye; Lim, Su Young; Park, Sung Hye; Kim, Jong-Hoon; Kim, Dong Gyu; Jung, Hee Won; Cho, Byung Kyu; Paek, Sun Ha.
In: Journal of Cancer Research and Clinical Oncology, Vol. 134, No. 2, 01.02.2008, p. 255-262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigation of molecular factors associated with malignant transformation of oligodendroglioma by proteomic study of a single case of rapid tumor progression
AU - Park, Chul Kee
AU - Kim, Jin Hyun
AU - Moon, Min Jeong
AU - Jung, Ji Hye
AU - Lim, Su Young
AU - Park, Sung Hye
AU - Kim, Jong-Hoon
AU - Kim, Dong Gyu
AU - Jung, Hee Won
AU - Cho, Byung Kyu
AU - Paek, Sun Ha
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Purpose: Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation. Methods: We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach. Results: Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue. Conclusions: Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
AB - Purpose: Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation. Methods: We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach. Results: Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue. Conclusions: Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
KW - Malignant transformation
KW - Mass spectrometry
KW - Oligodendroglioma
KW - Peroxiredoxin
KW - Rho GDP dissociation inhibitor
UR - http://www.scopus.com/inward/record.url?scp=36849048202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36849048202&partnerID=8YFLogxK
U2 - 10.1007/s00432-007-0282-1
DO - 10.1007/s00432-007-0282-1
M3 - Article
C2 - 17653765
AN - SCOPUS:36849048202
VL - 134
SP - 255
EP - 262
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
SN - 0171-5216
IS - 2
ER -