Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content

Xiaoping Li, Sang Wook Kim, Jung Suck Choi, Yoon Mi Lee, Cheol-Koo Lee, Bong Hwan Choi, Tae Hun Kim, Yang Il Choi, Jong Joo Kim, Kwan Suk Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Porcine chromosome 6 (SSC6) has been reported to have QTL affecting intramuscular fat content (IMF) in multiple populations. The objective of this study was to investigate the effect of FABP3 and LEPR genetic variations as well as their mRNA expression on the IMF trait in a three-generation of Korean native pig and Yorkshire crossed animals. Several polymorphisms of the FABP3 (HinfI, HaeIII and HinfI) were significantly associated with moisture, tenderness and flavor score (P < 0.05), and were used to construct haploytpes: haplotype 1 (-TCT-) increased the marbling and intramuscular fat content, however, haplotype 2 (-CCT-) decreased tenderness. The LEPR AvaII polymorphism showed significant association with moisture, intramuscular fat, cholesterol and flavor score (P < 0.05). The linkage analyses with six microsatellites mapped FABP3 gene in the interval between the markers Sw1129 and S0228 (Sw1129-11.7 cM-FABP3-9.1 cM-S0228), and the LEPR gene between the markers S0121 and Sw322 (S0121-7.5 cM-LEPR-28.5 cM-Sw322). QTL mapping suggested a significant QTL affecting Moisture (83 cM) and IMF (84 cM) located close to marker S0228. The gene expression results showed that in the loin muscle, both of the FABP3 and LEPR genes showed significantly higher expression in pigs with higher IMF%, however, in the backfat, only FABP3 showed differential expression between these two groups of pigs (significantly higher expression in pigs with lower IMF%) (P < 0.05). In the liver, both of these two genes did not show any difference between the high and low IMF% groups.

Original languageEnglish
Pages (from-to)3931-3939
Number of pages9
JournalMolecular Biology Reports
Volume37
Issue number8
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Swine
Fats
Messenger RNA
Genes
Haplotypes
Chromosomes, Human, Pair 6
Microsatellite Repeats
Cholesterol
Gene Expression
Muscles
Liver
Population

Keywords

  • FABP3
  • Gene expression
  • LEPR
  • Marbling
  • Pig
  • QTL
  • SNP

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content. / Li, Xiaoping; Kim, Sang Wook; Choi, Jung Suck; Lee, Yoon Mi; Lee, Cheol-Koo; Choi, Bong Hwan; Kim, Tae Hun; Choi, Yang Il; Kim, Jong Joo; Kim, Kwan Suk.

In: Molecular Biology Reports, Vol. 37, No. 8, 01.12.2010, p. 3931-3939.

Research output: Contribution to journalArticle

Li, Xiaoping ; Kim, Sang Wook ; Choi, Jung Suck ; Lee, Yoon Mi ; Lee, Cheol-Koo ; Choi, Bong Hwan ; Kim, Tae Hun ; Choi, Yang Il ; Kim, Jong Joo ; Kim, Kwan Suk. / Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content. In: Molecular Biology Reports. 2010 ; Vol. 37, No. 8. pp. 3931-3939.
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abstract = "Porcine chromosome 6 (SSC6) has been reported to have QTL affecting intramuscular fat content (IMF) in multiple populations. The objective of this study was to investigate the effect of FABP3 and LEPR genetic variations as well as their mRNA expression on the IMF trait in a three-generation of Korean native pig and Yorkshire crossed animals. Several polymorphisms of the FABP3 (HinfI, HaeIII and HinfI) were significantly associated with moisture, tenderness and flavor score (P < 0.05), and were used to construct haploytpes: haplotype 1 (-TCT-) increased the marbling and intramuscular fat content, however, haplotype 2 (-CCT-) decreased tenderness. The LEPR AvaII polymorphism showed significant association with moisture, intramuscular fat, cholesterol and flavor score (P < 0.05). The linkage analyses with six microsatellites mapped FABP3 gene in the interval between the markers Sw1129 and S0228 (Sw1129-11.7 cM-FABP3-9.1 cM-S0228), and the LEPR gene between the markers S0121 and Sw322 (S0121-7.5 cM-LEPR-28.5 cM-Sw322). QTL mapping suggested a significant QTL affecting Moisture (83 cM) and IMF (84 cM) located close to marker S0228. The gene expression results showed that in the loin muscle, both of the FABP3 and LEPR genes showed significantly higher expression in pigs with higher IMF{\%}, however, in the backfat, only FABP3 showed differential expression between these two groups of pigs (significantly higher expression in pigs with lower IMF{\%}) (P < 0.05). In the liver, both of these two genes did not show any difference between the high and low IMF{\%} groups.",
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AU - Kim, Sang Wook

AU - Choi, Jung Suck

AU - Lee, Yoon Mi

AU - Lee, Cheol-Koo

AU - Choi, Bong Hwan

AU - Kim, Tae Hun

AU - Choi, Yang Il

AU - Kim, Jong Joo

AU - Kim, Kwan Suk

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