Abstract
Background: Various types of external stress cause the skin and central neuroendocrine system to express corticotropin-releasing hormone (CRH)-proopiomelanocortin (POMC) axis-related hormones. However, the precise role of the CRH-POMC axis-related hormones in various skin tumours is unclear. Objectives: This study examined expression patterns of the CRH-POMC axis-related hormones in skin tumours. Methods: The production of CRH, adrenocorticotropic hormone (ACTH) and α-melanocyte-stimulating hormone (α-MSH) in various tumour cell lines including HaCaT and primary keratinocytes was examined using an enzyme-linked immunosorbent assay. Immunohistochemical analysis of the skin tumours was also performed. Results: CRH, ACTH and α-MSH were strongly expressed in malignant skin tumour cell lines such as G-361 and DX-3 (both malignant melanoma, MM). However, normal and haematological malignancy cell lines did not express the CRH-POMC axis-related hormones. Immunohistochemical analysis of the skin tumours showed that MM (80%), squamous cell carcinoma (SCC, 70%) and basal cell carcinoma (BCC, 10%) had strong immunoreactivity (++/+++) for CRH. Strong ACTH and α-MSH expression was observed in MM (70% and 50%, respectively), SCC (80% and 60%, respectively) and BCC (70% and 50%, respectively). Conclusions: We report that an increase in the level of the CRH-POMC axis-related hormones is associated with malignant skin tumours such as MM. These results highlight the importance of the CRH-POMC axis-related hormones in the malignant tendency of skin tumours.
| Original language | English |
|---|---|
| Pages (from-to) | 910-915 |
| Number of pages | 6 |
| Journal | British Journal of Dermatology |
| Volume | 155 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 Nov 1 |
| Externally published | Yes |
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Keywords
- α-melanocyte- stimulating hormone
- Adrenocorticotropic hormone
- Corticotropin-releasing hormone
- Corticotropin-releasing hormone-proopiomelanocortin axis
- Skin tumour
ASJC Scopus subject areas
- Dermatology
Cite this
Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. / Kim, M. H.; Cho, Dae Ho; Kim, H. J.; Chong, S. J.; Lee, K. H.; Yu, D. S.; Park, C. J.; Lee, J. Y.; Cho, B. K.; Park, H. J.
In: British Journal of Dermatology, Vol. 155, No. 5, 01.11.2006, p. 910-915.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours
AU - Kim, M. H.
AU - Cho, Dae Ho
AU - Kim, H. J.
AU - Chong, S. J.
AU - Lee, K. H.
AU - Yu, D. S.
AU - Park, C. J.
AU - Lee, J. Y.
AU - Cho, B. K.
AU - Park, H. J.
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Background: Various types of external stress cause the skin and central neuroendocrine system to express corticotropin-releasing hormone (CRH)-proopiomelanocortin (POMC) axis-related hormones. However, the precise role of the CRH-POMC axis-related hormones in various skin tumours is unclear. Objectives: This study examined expression patterns of the CRH-POMC axis-related hormones in skin tumours. Methods: The production of CRH, adrenocorticotropic hormone (ACTH) and α-melanocyte-stimulating hormone (α-MSH) in various tumour cell lines including HaCaT and primary keratinocytes was examined using an enzyme-linked immunosorbent assay. Immunohistochemical analysis of the skin tumours was also performed. Results: CRH, ACTH and α-MSH were strongly expressed in malignant skin tumour cell lines such as G-361 and DX-3 (both malignant melanoma, MM). However, normal and haematological malignancy cell lines did not express the CRH-POMC axis-related hormones. Immunohistochemical analysis of the skin tumours showed that MM (80%), squamous cell carcinoma (SCC, 70%) and basal cell carcinoma (BCC, 10%) had strong immunoreactivity (++/+++) for CRH. Strong ACTH and α-MSH expression was observed in MM (70% and 50%, respectively), SCC (80% and 60%, respectively) and BCC (70% and 50%, respectively). Conclusions: We report that an increase in the level of the CRH-POMC axis-related hormones is associated with malignant skin tumours such as MM. These results highlight the importance of the CRH-POMC axis-related hormones in the malignant tendency of skin tumours.
AB - Background: Various types of external stress cause the skin and central neuroendocrine system to express corticotropin-releasing hormone (CRH)-proopiomelanocortin (POMC) axis-related hormones. However, the precise role of the CRH-POMC axis-related hormones in various skin tumours is unclear. Objectives: This study examined expression patterns of the CRH-POMC axis-related hormones in skin tumours. Methods: The production of CRH, adrenocorticotropic hormone (ACTH) and α-melanocyte-stimulating hormone (α-MSH) in various tumour cell lines including HaCaT and primary keratinocytes was examined using an enzyme-linked immunosorbent assay. Immunohistochemical analysis of the skin tumours was also performed. Results: CRH, ACTH and α-MSH were strongly expressed in malignant skin tumour cell lines such as G-361 and DX-3 (both malignant melanoma, MM). However, normal and haematological malignancy cell lines did not express the CRH-POMC axis-related hormones. Immunohistochemical analysis of the skin tumours showed that MM (80%), squamous cell carcinoma (SCC, 70%) and basal cell carcinoma (BCC, 10%) had strong immunoreactivity (++/+++) for CRH. Strong ACTH and α-MSH expression was observed in MM (70% and 50%, respectively), SCC (80% and 60%, respectively) and BCC (70% and 50%, respectively). Conclusions: We report that an increase in the level of the CRH-POMC axis-related hormones is associated with malignant skin tumours such as MM. These results highlight the importance of the CRH-POMC axis-related hormones in the malignant tendency of skin tumours.
KW - α-melanocyte- stimulating hormone
KW - Adrenocorticotropic hormone
KW - Corticotropin-releasing hormone
KW - Corticotropin-releasing hormone-proopiomelanocortin axis
KW - Skin tumour
UR - http://www.scopus.com/inward/record.url?scp=33749857509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749857509&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2133.2006.07442.x
DO - 10.1111/j.1365-2133.2006.07442.x
M3 - Article
C2 - 17034518
AN - SCOPUS:33749857509
VL - 155
SP - 910
EP - 915
JO - British Journal of Dermatology
JF - British Journal of Dermatology
SN - 0007-0963
IS - 5
ER -