Investigation of the elasticity of a cisplatin-DNA adduct via single-molecule measurements and bimodal modeling

Nam Kyung Lee, Jin Sung Park, Albert Johner, Sergei Obukhov, Ju Yong Hyon, Kyoung Jin Lee, Seok Cheol Hong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cisplatin has been known as an anticancer drug for a long time. It is therapeutically active upon binding to DNA. A double-bound cisplatin bends DNA into a localized kink. We model the elastic properties of cisplatin-DNA adducts at moderate tension (<6pN). It is shown that from the mechanical point of view the action of cisplatin can be revealed by reduced persistence length. We derived two expressions for the persistence length, which apply in the linear-response and the strong-force regimes, respectively. Experimental data for DNA adducts stretched by magnetic tweezers are consistently fitted by these expressions. This allows us to estimate the degree of platination at various salt concentrations.

Original languageEnglish
Article number041921
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume79
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1

Fingerprint

Cisplatin
Bimodal
adducts
Elasticity
deoxyribonucleic acid
elastic properties
Modeling
Persistence
molecules
Linear Response
Elastic Properties
Kink
Salt
Drugs
drugs
Experimental Data
salts
estimates
Estimate

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Statistical and Nonlinear Physics
  • Statistics and Probability

Cite this

Investigation of the elasticity of a cisplatin-DNA adduct via single-molecule measurements and bimodal modeling. / Lee, Nam Kyung; Park, Jin Sung; Johner, Albert; Obukhov, Sergei; Hyon, Ju Yong; Lee, Kyoung Jin; Hong, Seok Cheol.

In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 79, No. 4, 041921, 01.04.2009.

Research output: Contribution to journalArticle

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