Investigation of the growth rate change in recombinant BCG which was cloned Mycobacterium tuberculosis adenylate kinase mutation gene or human muscle-type adenylate kinase synthetic gene

Seung Heon Lee, Hyo Joon Kim, Young Kil Park, Gill Han Bai

Research output: Contribution to journalArticle

Abstract

Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalTuberculosis and Respiratory Diseases
Volume60
Issue number2
DOIs
Publication statusPublished - 2006 Jan 1
Externally publishedYes

Fingerprint

Synthetic Genes
Adenylate Kinase
Mycobacterium bovis
Mycobacterium tuberculosis
Muscles
Mutation
Growth
Genes
Metabolome
Mycobacterium
Cell Survival
Nucleotides
Maintenance
Cell Proliferation
Escherichia coli

Keywords

  • Adenylate kinase
  • BCG
  • Growth rate
  • Mutation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

@article{da704cb982514dac84f7e82e2b3944be,
title = "Investigation of the growth rate change in recombinant BCG which was cloned Mycobacterium tuberculosis adenylate kinase mutation gene or human muscle-type adenylate kinase synthetic gene",
abstract = "Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.",
keywords = "Adenylate kinase, BCG, Growth rate, Mutation",
author = "Lee, {Seung Heon} and Kim, {Hyo Joon} and Park, {Young Kil} and Bai, {Gill Han}",
year = "2006",
month = "1",
day = "1",
doi = "10.4046/trd.2006.60.2.187",
language = "English",
volume = "60",
pages = "187--193",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
number = "2",

}

TY - JOUR

T1 - Investigation of the growth rate change in recombinant BCG which was cloned Mycobacterium tuberculosis adenylate kinase mutation gene or human muscle-type adenylate kinase synthetic gene

AU - Lee, Seung Heon

AU - Kim, Hyo Joon

AU - Park, Young Kil

AU - Bai, Gill Han

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.

AB - Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.

KW - Adenylate kinase

KW - BCG

KW - Growth rate

KW - Mutation

UR - http://www.scopus.com/inward/record.url?scp=33646544705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646544705&partnerID=8YFLogxK

U2 - 10.4046/trd.2006.60.2.187

DO - 10.4046/trd.2006.60.2.187

M3 - Article

VL - 60

SP - 187

EP - 193

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

SN - 1738-3536

IS - 2

ER -