Abstract
Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.
| Original language | English |
|---|---|
| Pages (from-to) | 187-193 |
| Number of pages | 7 |
| Journal | Tuberculosis and Respiratory Diseases |
| Volume | 60 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2006 Jan 1 |
| Externally published | Yes |
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Keywords
- Adenylate kinase
- BCG
- Growth rate
- Mutation
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Infectious Diseases
Cite this
Investigation of the growth rate change in recombinant BCG which was cloned Mycobacterium tuberculosis adenylate kinase mutation gene or human muscle-type adenylate kinase synthetic gene. / Lee, Seung Heon; Kim, Hyo Joon; Park, Young Kil; Bai, Gill Han.
In: Tuberculosis and Respiratory Diseases, Vol. 60, No. 2, 01.01.2006, p. 187-193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigation of the growth rate change in recombinant BCG which was cloned Mycobacterium tuberculosis adenylate kinase mutation gene or human muscle-type adenylate kinase synthetic gene
AU - Lee, Seung Heon
AU - Kim, Hyo Joon
AU - Park, Young Kil
AU - Bai, Gill Han
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.
AB - Background: Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method: Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result: There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion: The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.
KW - Adenylate kinase
KW - BCG
KW - Growth rate
KW - Mutation
UR - http://www.scopus.com/inward/record.url?scp=33646544705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646544705&partnerID=8YFLogxK
U2 - 10.4046/trd.2006.60.2.187
DO - 10.4046/trd.2006.60.2.187
M3 - Article
AN - SCOPUS:33646544705
VL - 60
SP - 187
EP - 193
JO - Tuberculosis and Respiratory Diseases
JF - Tuberculosis and Respiratory Diseases
SN - 1738-3536
IS - 2
ER -