Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma

Yong Kang Sea Yong Kang, Jeong Shim Jae Jeong Shim, Yun Cho Jae Yun Cho, Hwan Kwon Young Hwan Kwon, Yong Lee Seung Yong Lee, Je Hyeong Kim, Youb Lee Sang Youb Lee, Ra Lee So Ra Lee, Ae Han Seon Ae Han, Gyum Kim Han Gyum Kim, Ho Kang Kyung Ho Kang, Hwa Yoo Se Hwa Yoo, Ho In Kwang Ho In

Research output: Contribution to journalArticle

Abstract

Background: Interleukin-4 plays an important role in pathogenesis of asthma, especially in developing atopy by means of switching B lymphocytes to produce IgE. It has been shown that there is polymorphism in the Interleukin- 4 promoter region, transversion of cytosine to thymine at -598 from translation initiation site of IL-4 gene. There has also been quite a few works to reveal the role of the polymorphism of IL-4 gene in patients with asthma. We performed this investigation to determine the role of the polymorphism in the severity of symptoms of patients with asthma. We also examined the frequency and the type of the polymorphism in asthmatics compared with non-asthmatics as well. Method: The subjects enrolled in this study were 49 asthmatics and 33 non-asthmatics. All the asthmatics were classified as mild and moderate to severe by the NHLBI/WHO Workshop. DNA from beth asthmatics and non-asthmatics was extracted, then performed ARMS(Amplification Refractory Mutation System) as well as RFLP using BsmFl restriction enzyme in order to confirm the polymorphism of IL-4 gene. Results: There was no significant difference in the occurrence of polymorphism of the IL-4 promoter sequence between asthma and non-asthma groups (P=0.7). Among these with polymorphisms, the number of C/C type was slightly more than C/T type in beth asthmatics and non-asthmatics, 26 vs 21 in asthmatics and 18 vs 15 in non-asthmatics, which was, however, insignificant statistically. No significant relationship between the severity of asthma and the polymorphism was found (P = 0.7). Conclusion: There was no significant difference between the severity of asthma and the IL-4 promoter polymorphism(P=0.709). Interestingly, the frequency of the polymorphism in both asthmatics as well as non-asthmatics was found to be even higher than that occurred in Caucasians. However, no significant difference in the frequency of the polymorphism was found in both groups.

Original languageEnglish
Pages (from-to)529-535
Number of pages7
JournalTuberculosis and Respiratory Diseases
Volume45
Issue number3
Publication statusPublished - 1998 Jan 1

Fingerprint

Interleukin-4
Asthma
Genes
National Heart, Lung, and Blood Institute (U.S.)
Thymine
Cytosine
Genetic Promoter Regions
Restriction Fragment Length Polymorphisms
Immunoglobulin E
B-Lymphocytes
Education
Mutation
DNA
Enzymes

Keywords

  • Asthma
  • Interleukin-4
  • Polymorphism
  • Promoter

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Sea Yong Kang, Y. K., Jae Jeong Shim, J. S., Jae Yun Cho, Y. C., Young Hwan Kwon, H. K., Seung Yong Lee, Y. L., Kim, J. H., ... Kwang Ho In, H. I. (1998). Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma. Tuberculosis and Respiratory Diseases, 45(3), 529-535.

Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma. / Sea Yong Kang, Yong Kang; Jae Jeong Shim, Jeong Shim; Jae Yun Cho, Yun Cho; Young Hwan Kwon, Hwan Kwon; Seung Yong Lee, Yong Lee; Kim, Je Hyeong; Sang Youb Lee, Youb Lee; So Ra Lee, Ra Lee; Seon Ae Han, Ae Han; Han Gyum Kim, Gyum Kim; Kyung Ho Kang, Ho Kang; Se Hwa Yoo, Hwa Yoo; Kwang Ho In, Ho In.

In: Tuberculosis and Respiratory Diseases, Vol. 45, No. 3, 01.01.1998, p. 529-535.

Research output: Contribution to journalArticle

Sea Yong Kang, YK, Jae Jeong Shim, JS, Jae Yun Cho, YC, Young Hwan Kwon, HK, Seung Yong Lee, YL, Kim, JH, Sang Youb Lee, YL, So Ra Lee, RL, Seon Ae Han, AH, Han Gyum Kim, GK, Kyung Ho Kang, HK, Se Hwa Yoo, HY & Kwang Ho In, HI 1998, 'Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma', Tuberculosis and Respiratory Diseases, vol. 45, no. 3, pp. 529-535.
Sea Yong Kang YK, Jae Jeong Shim JS, Jae Yun Cho YC, Young Hwan Kwon HK, Seung Yong Lee YL, Kim JH et al. Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma. Tuberculosis and Respiratory Diseases. 1998 Jan 1;45(3):529-535.
Sea Yong Kang, Yong Kang ; Jae Jeong Shim, Jeong Shim ; Jae Yun Cho, Yun Cho ; Young Hwan Kwon, Hwan Kwon ; Seung Yong Lee, Yong Lee ; Kim, Je Hyeong ; Sang Youb Lee, Youb Lee ; So Ra Lee, Ra Lee ; Seon Ae Han, Ae Han ; Han Gyum Kim, Gyum Kim ; Kyung Ho Kang, Ho Kang ; Se Hwa Yoo, Hwa Yoo ; Kwang Ho In, Ho In. / Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma. In: Tuberculosis and Respiratory Diseases. 1998 ; Vol. 45, No. 3. pp. 529-535.
@article{501e171dd60342cbb922a9d21f4df3b4,
title = "Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma",
abstract = "Background: Interleukin-4 plays an important role in pathogenesis of asthma, especially in developing atopy by means of switching B lymphocytes to produce IgE. It has been shown that there is polymorphism in the Interleukin- 4 promoter region, transversion of cytosine to thymine at -598 from translation initiation site of IL-4 gene. There has also been quite a few works to reveal the role of the polymorphism of IL-4 gene in patients with asthma. We performed this investigation to determine the role of the polymorphism in the severity of symptoms of patients with asthma. We also examined the frequency and the type of the polymorphism in asthmatics compared with non-asthmatics as well. Method: The subjects enrolled in this study were 49 asthmatics and 33 non-asthmatics. All the asthmatics were classified as mild and moderate to severe by the NHLBI/WHO Workshop. DNA from beth asthmatics and non-asthmatics was extracted, then performed ARMS(Amplification Refractory Mutation System) as well as RFLP using BsmFl restriction enzyme in order to confirm the polymorphism of IL-4 gene. Results: There was no significant difference in the occurrence of polymorphism of the IL-4 promoter sequence between asthma and non-asthma groups (P=0.7). Among these with polymorphisms, the number of C/C type was slightly more than C/T type in beth asthmatics and non-asthmatics, 26 vs 21 in asthmatics and 18 vs 15 in non-asthmatics, which was, however, insignificant statistically. No significant relationship between the severity of asthma and the polymorphism was found (P = 0.7). Conclusion: There was no significant difference between the severity of asthma and the IL-4 promoter polymorphism(P=0.709). Interestingly, the frequency of the polymorphism in both asthmatics as well as non-asthmatics was found to be even higher than that occurred in Caucasians. However, no significant difference in the frequency of the polymorphism was found in both groups.",
keywords = "Asthma, Interleukin-4, Polymorphism, Promoter",
author = "{Sea Yong Kang}, {Yong Kang} and {Jae Jeong Shim}, {Jeong Shim} and {Jae Yun Cho}, {Yun Cho} and {Young Hwan Kwon}, {Hwan Kwon} and {Seung Yong Lee}, {Yong Lee} and Kim, {Je Hyeong} and {Sang Youb Lee}, {Youb Lee} and {So Ra Lee}, {Ra Lee} and {Seon Ae Han}, {Ae Han} and {Han Gyum Kim}, {Gyum Kim} and {Kyung Ho Kang}, {Ho Kang} and {Se Hwa Yoo}, {Hwa Yoo} and {Kwang Ho In}, {Ho In}",
year = "1998",
month = "1",
day = "1",
language = "English",
volume = "45",
pages = "529--535",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
number = "3",

}

TY - JOUR

T1 - Investigation of the relationship between interleukin-4 promoter polymorphism and severity of patients with bronchial asthma

AU - Sea Yong Kang, Yong Kang

AU - Jae Jeong Shim, Jeong Shim

AU - Jae Yun Cho, Yun Cho

AU - Young Hwan Kwon, Hwan Kwon

AU - Seung Yong Lee, Yong Lee

AU - Kim, Je Hyeong

AU - Sang Youb Lee, Youb Lee

AU - So Ra Lee, Ra Lee

AU - Seon Ae Han, Ae Han

AU - Han Gyum Kim, Gyum Kim

AU - Kyung Ho Kang, Ho Kang

AU - Se Hwa Yoo, Hwa Yoo

AU - Kwang Ho In, Ho In

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Background: Interleukin-4 plays an important role in pathogenesis of asthma, especially in developing atopy by means of switching B lymphocytes to produce IgE. It has been shown that there is polymorphism in the Interleukin- 4 promoter region, transversion of cytosine to thymine at -598 from translation initiation site of IL-4 gene. There has also been quite a few works to reveal the role of the polymorphism of IL-4 gene in patients with asthma. We performed this investigation to determine the role of the polymorphism in the severity of symptoms of patients with asthma. We also examined the frequency and the type of the polymorphism in asthmatics compared with non-asthmatics as well. Method: The subjects enrolled in this study were 49 asthmatics and 33 non-asthmatics. All the asthmatics were classified as mild and moderate to severe by the NHLBI/WHO Workshop. DNA from beth asthmatics and non-asthmatics was extracted, then performed ARMS(Amplification Refractory Mutation System) as well as RFLP using BsmFl restriction enzyme in order to confirm the polymorphism of IL-4 gene. Results: There was no significant difference in the occurrence of polymorphism of the IL-4 promoter sequence between asthma and non-asthma groups (P=0.7). Among these with polymorphisms, the number of C/C type was slightly more than C/T type in beth asthmatics and non-asthmatics, 26 vs 21 in asthmatics and 18 vs 15 in non-asthmatics, which was, however, insignificant statistically. No significant relationship between the severity of asthma and the polymorphism was found (P = 0.7). Conclusion: There was no significant difference between the severity of asthma and the IL-4 promoter polymorphism(P=0.709). Interestingly, the frequency of the polymorphism in both asthmatics as well as non-asthmatics was found to be even higher than that occurred in Caucasians. However, no significant difference in the frequency of the polymorphism was found in both groups.

AB - Background: Interleukin-4 plays an important role in pathogenesis of asthma, especially in developing atopy by means of switching B lymphocytes to produce IgE. It has been shown that there is polymorphism in the Interleukin- 4 promoter region, transversion of cytosine to thymine at -598 from translation initiation site of IL-4 gene. There has also been quite a few works to reveal the role of the polymorphism of IL-4 gene in patients with asthma. We performed this investigation to determine the role of the polymorphism in the severity of symptoms of patients with asthma. We also examined the frequency and the type of the polymorphism in asthmatics compared with non-asthmatics as well. Method: The subjects enrolled in this study were 49 asthmatics and 33 non-asthmatics. All the asthmatics were classified as mild and moderate to severe by the NHLBI/WHO Workshop. DNA from beth asthmatics and non-asthmatics was extracted, then performed ARMS(Amplification Refractory Mutation System) as well as RFLP using BsmFl restriction enzyme in order to confirm the polymorphism of IL-4 gene. Results: There was no significant difference in the occurrence of polymorphism of the IL-4 promoter sequence between asthma and non-asthma groups (P=0.7). Among these with polymorphisms, the number of C/C type was slightly more than C/T type in beth asthmatics and non-asthmatics, 26 vs 21 in asthmatics and 18 vs 15 in non-asthmatics, which was, however, insignificant statistically. No significant relationship between the severity of asthma and the polymorphism was found (P = 0.7). Conclusion: There was no significant difference between the severity of asthma and the IL-4 promoter polymorphism(P=0.709). Interestingly, the frequency of the polymorphism in both asthmatics as well as non-asthmatics was found to be even higher than that occurred in Caucasians. However, no significant difference in the frequency of the polymorphism was found in both groups.

KW - Asthma

KW - Interleukin-4

KW - Polymorphism

KW - Promoter

UR - http://www.scopus.com/inward/record.url?scp=0031752642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031752642&partnerID=8YFLogxK

M3 - Article

VL - 45

SP - 529

EP - 535

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

SN - 1738-3536

IS - 3

ER -