Involvement of CLOCK: BMALI heterodimer in serum-responsive mPerl induction

Hosung Jung, Youngshik Choe, Hyunjung Kim, Noheon Park, Gi Hoon Son, Inkoo Khang, Kyungjin Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A rapid induction of mouse periodl (mPerl) gene expression is supposed to be critical in the clock gene regulation, especially in the phase resetting of the clock, but its molecular mechanism is poorly understood. Based on the previous finding that the process does not involve de novo synthesis of proteins, we postulated the involvement of CLOCK:BMALI heterodimer, a positive regulator of circadian oscillator, in the rapid induction of mPerl transcription. To test this hypothesis, we utilized CLOCKδ19, a dominant-negative mutant, to suppress the function of CLOCK:BMALI in vitro. Serum-evoked rapid increases of mPerl mRNA expression and promoter activity were significantly blunted when CLOCK:BMALI function was interfered with. Furthermore, DNA binding activity of CLOCK:BMALI heterodimer to five E-boxes of mPerl promoter markedly increased shortly after serum shock. Taken together, these results suggest that CLOCK:BMALI heterodimer is not only a core component of negative feedback loop driving circadian oscillator, but also involved in the rapid induction of mPerl during phase resetting of the clock.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalNeuroReport
Volume14
Issue number1
DOIs
Publication statusPublished - 2003 Jan 20
Externally publishedYes

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Keywords

  • Bmall
  • Clock
  • Mouse period I (mPerl)
  • NIH-3T3 mouse fibroblast cells
  • Serum shock
  • Transcriptional regulation

ASJC Scopus subject areas

  • Neuroscience(all)

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