Abstract
The present study investigates the possible mechanism responsible for the neurotoxicity of D,L-homocysteine in primary culture of rat cerebellar granule cells. Neurotoxicity was assessed by measuring the amount of lactate dehydrogenase released from the cells following homocysteine treatment. D,L-Homocysteine (>300 μM; 16-22 h) induced the release of lactate dehydrogenase from the cells in a concentration-dependent manner. The N-methyl-D-aspartate (NMDA) antagonist (±)-2-amino-5-phosphonopentanoic acid (APV) partially blocked the homocysteine-mediated neurotoxicity. However, the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) did not block the homocysteine-mediated toxicity. The homocysteine-mediated neurotoxicity was mostly prevented by the co-administration of superoxide dismutase and catalase or catalase alone. The results suggest that homocysteine induces neuronal cell death by stimulating NMDA receptor as well as by producing free radicals.
| Original language | English |
|---|---|
| Pages (from-to) | 117-120 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 216 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1996 Sep 27 |
| Externally published | Yes |
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Keywords
- Cerebellar granule cell
- Free radical
- Homocysteine
- Lactate dehydrogenase
- N-Methyl-D-aspartate
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Involvement of N-methyl-D-aspartate receptor and free radical in homocysteine-mediated toxicity on rat cerebellar granule cells in culture. / Kim, Won-Ki; Pae, Young Sook.
In: Neuroscience Letters, Vol. 216, No. 2, 27.09.1996, p. 117-120.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of N-methyl-D-aspartate receptor and free radical in homocysteine-mediated toxicity on rat cerebellar granule cells in culture
AU - Kim, Won-Ki
AU - Pae, Young Sook
PY - 1996/9/27
Y1 - 1996/9/27
N2 - The present study investigates the possible mechanism responsible for the neurotoxicity of D,L-homocysteine in primary culture of rat cerebellar granule cells. Neurotoxicity was assessed by measuring the amount of lactate dehydrogenase released from the cells following homocysteine treatment. D,L-Homocysteine (>300 μM; 16-22 h) induced the release of lactate dehydrogenase from the cells in a concentration-dependent manner. The N-methyl-D-aspartate (NMDA) antagonist (±)-2-amino-5-phosphonopentanoic acid (APV) partially blocked the homocysteine-mediated neurotoxicity. However, the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) did not block the homocysteine-mediated toxicity. The homocysteine-mediated neurotoxicity was mostly prevented by the co-administration of superoxide dismutase and catalase or catalase alone. The results suggest that homocysteine induces neuronal cell death by stimulating NMDA receptor as well as by producing free radicals.
AB - The present study investigates the possible mechanism responsible for the neurotoxicity of D,L-homocysteine in primary culture of rat cerebellar granule cells. Neurotoxicity was assessed by measuring the amount of lactate dehydrogenase released from the cells following homocysteine treatment. D,L-Homocysteine (>300 μM; 16-22 h) induced the release of lactate dehydrogenase from the cells in a concentration-dependent manner. The N-methyl-D-aspartate (NMDA) antagonist (±)-2-amino-5-phosphonopentanoic acid (APV) partially blocked the homocysteine-mediated neurotoxicity. However, the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) did not block the homocysteine-mediated toxicity. The homocysteine-mediated neurotoxicity was mostly prevented by the co-administration of superoxide dismutase and catalase or catalase alone. The results suggest that homocysteine induces neuronal cell death by stimulating NMDA receptor as well as by producing free radicals.
KW - Cerebellar granule cell
KW - Free radical
KW - Homocysteine
KW - Lactate dehydrogenase
KW - N-Methyl-D-aspartate
UR - http://www.scopus.com/inward/record.url?scp=0030604041&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030604041&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(96)13011-1
DO - 10.1016/S0304-3940(96)13011-1
M3 - Article
C2 - 8904797
AN - SCOPUS:0030604041
VL - 216
SP - 117
EP - 120
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -