Involvement of protein kinase C and Rho GTPase in the nuclear signalling pathway by transforming growth factor-β1 in rat-2 fibroblast cells

Sung E. Choi, Eui Yul Choi, Pyeung Hyeun Kim, Jae Hong Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The transforming growth factor (TGF)-β signal-transduction cascade from the cell membrane to the nuclear target is poorly characterised. Here we report that treatment with TGF-β1 induces the levels of endogenous c-fos mRNA in Rat-2 fibroblast cells. In addition, by transient transfection analysis, TGF-β1 was shown to stimulate c-fos serum response element (SRE)-driven reporter gene activity in a dose- and time-dependent manner, suggesting that SRE is one of the nuclear targets of TGF-β1. To understand the signalling cascade by which TGF-β1 mediates the transactivation of c-fos SRE, cells were either pre-treated with various inhibitors or co-transfected with expression plasmids encoding inhibitory proteins for Rho GTPase together with the SRE-luciferase reporter gene. Our results showed that an inhibition of protein kinase C (PKC) or RhoA selectively repressed the stimulation of c-fos SRE by TGF-β1, implying the possible roles of PKC and RhoA GTPase in TGF-β1-induced signalling to c-fos SRE. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalCellular Signalling
Volume11
Issue number1
DOIs
Publication statusPublished - 1999 Jan
Externally publishedYes

Keywords

  • MAP kinase
  • PKC
  • Rho
  • SRE
  • Signal transduction
  • TGF-β
  • c-fos

ASJC Scopus subject areas

  • Cell Biology

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