Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice

Ediriweera Ps Chandana, Yasuhiro Maeda, Akihiko Ueda, Hiroshi Kiyonari, Naoko Oshima, Mako Yamamoto, Shunya Kondo, Jun Seo Oh, Rei Takahashi, Yoko Yoshida, Satoshi Kawashima, David B. Alexander, Hitoshi Kitayama, Chiaki Takahashi, Yasuhiko Tabata, Tomoko Matsuzaki, Makoto Noda

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background. Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear. Results. We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos. Conclusions. Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyornic tissues.

Original languageEnglish
Article number84
JournalBMC Developmental Biology
Volume10
DOIs
Publication statusPublished - 2010 Aug 9

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Tumor Suppressor Proteins
Blood Vessels
Mothers
Intussusception
Metalloproteases
Small Interfering RNA
Uterus
Embryonic Structures
Phenotype
Membranes
Vascular Remodeling

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Chandana, E. P., Maeda, Y., Ueda, A., Kiyonari, H., Oshima, N., Yamamoto, M., ... Noda, M. (2010). Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. BMC Developmental Biology, 10, [84]. https://doi.org/10.1186/1471-213X-10-84

Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. / Chandana, Ediriweera Ps; Maeda, Yasuhiro; Ueda, Akihiko; Kiyonari, Hiroshi; Oshima, Naoko; Yamamoto, Mako; Kondo, Shunya; Oh, Jun Seo; Takahashi, Rei; Yoshida, Yoko; Kawashima, Satoshi; Alexander, David B.; Kitayama, Hitoshi; Takahashi, Chiaki; Tabata, Yasuhiko; Matsuzaki, Tomoko; Noda, Makoto.

In: BMC Developmental Biology, Vol. 10, 84, 09.08.2010.

Research output: Contribution to journalArticle

Chandana, EP, Maeda, Y, Ueda, A, Kiyonari, H, Oshima, N, Yamamoto, M, Kondo, S, Oh, JS, Takahashi, R, Yoshida, Y, Kawashima, S, Alexander, DB, Kitayama, H, Takahashi, C, Tabata, Y, Matsuzaki, T & Noda, M 2010, 'Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice', BMC Developmental Biology, vol. 10, 84. https://doi.org/10.1186/1471-213X-10-84
Chandana, Ediriweera Ps ; Maeda, Yasuhiro ; Ueda, Akihiko ; Kiyonari, Hiroshi ; Oshima, Naoko ; Yamamoto, Mako ; Kondo, Shunya ; Oh, Jun Seo ; Takahashi, Rei ; Yoshida, Yoko ; Kawashima, Satoshi ; Alexander, David B. ; Kitayama, Hitoshi ; Takahashi, Chiaki ; Tabata, Yasuhiko ; Matsuzaki, Tomoko ; Noda, Makoto. / Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. In: BMC Developmental Biology. 2010 ; Vol. 10.
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