Abstract
Background. Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear. Results. We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos. Conclusions. Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyornic tissues.
| Original language | English |
|---|---|
| Article number | 84 |
| Journal | BMC Developmental Biology |
| Volume | 10 |
| DOIs | |
| Publication status | Published - 2010 Aug 9 |
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ASJC Scopus subject areas
- Developmental Biology
Cite this
Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. / Chandana, Ediriweera Ps; Maeda, Yasuhiro; Ueda, Akihiko; Kiyonari, Hiroshi; Oshima, Naoko; Yamamoto, Mako; Kondo, Shunya; Oh, Jun Seo; Takahashi, Rei; Yoshida, Yoko; Kawashima, Satoshi; Alexander, David B.; Kitayama, Hitoshi; Takahashi, Chiaki; Tabata, Yasuhiko; Matsuzaki, Tomoko; Noda, Makoto.
In: BMC Developmental Biology, Vol. 10, 84, 09.08.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice
AU - Chandana, Ediriweera Ps
AU - Maeda, Yasuhiro
AU - Ueda, Akihiko
AU - Kiyonari, Hiroshi
AU - Oshima, Naoko
AU - Yamamoto, Mako
AU - Kondo, Shunya
AU - Oh, Jun Seo
AU - Takahashi, Rei
AU - Yoshida, Yoko
AU - Kawashima, Satoshi
AU - Alexander, David B.
AU - Kitayama, Hitoshi
AU - Takahashi, Chiaki
AU - Tabata, Yasuhiko
AU - Matsuzaki, Tomoko
AU - Noda, Makoto
PY - 2010/8/9
Y1 - 2010/8/9
N2 - Background. Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear. Results. We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos. Conclusions. Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyornic tissues.
AB - Background. Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear. Results. We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos. Conclusions. Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyornic tissues.
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UR - http://www.scopus.com/inward/citedby.url?scp=77955188882&partnerID=8YFLogxK
U2 - 10.1186/1471-213X-10-84
DO - 10.1186/1471-213X-10-84
M3 - Article
C2 - 20691046
AN - SCOPUS:77955188882
VL - 10
JO - BMC Developmental Biology
JF - BMC Developmental Biology
SN - 1471-213X
M1 - 84
ER -