Ion concentration polarization for pre-concentration of biological samples without pH change

Youngkyu Cho, Junghyo Yoon, David Wonbin Lim, Jaehoon Kim, Jeong Hoon Lee, Seok Chung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this paper, a method was developed for pre-concentrating large-volume biological samples for subsequent analysis. We previously developed another pre-concentration device, but it unfortunately altered the pH of the sample when an electric field was applied to the sample reservoir. Changes in the pH are not suitable for subsequent antibody-antigen reactions because of the stability issues that arise based on the target molecule's isoelectric point (pI). Here, this problem was overcome using ion concentration polarization (ICP) with a cation selective membrane (Nafion). Phosphate buffered saline was used as a test solution for the sample. The sample was contained in a reservoir that was not affected by the electric field, and an ICP barrier was formed in front of the reservoir. This device could concentrate microliter-scale samples without changing the pH because the biomolecules were blocked from passing through the ICP barrier while the sample (phosphate buffered saline) was drained. A 40 μL sample was successfully pre-concentrated to 20 μL in a single channel device and 10 μL in a dual channel device, resulting in 2.1-fold and 3.3-fold increases, respectively, in influenza hemagglutinin concentrations. These changes in the concentration were confirmed by ELISA.

Original languageEnglish
Pages (from-to)6510-6514
Number of pages5
JournalAnalyst
Volume141
Issue number24
DOIs
Publication statusPublished - 2016 Dec 21

Fingerprint

polarization
Ions
Polarization
Equipment and Supplies
ion
electric field
Phosphates
Electric fields
Antigen-antibody reactions
phosphate
fold
influenza
Hemagglutinins
Biomolecules
Antigen-Antibody Reactions
antigen
antibody
Cations
Isoelectric Point
cation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Ion concentration polarization for pre-concentration of biological samples without pH change. / Cho, Youngkyu; Yoon, Junghyo; Lim, David Wonbin; Kim, Jaehoon; Lee, Jeong Hoon; Chung, Seok.

In: Analyst, Vol. 141, No. 24, 21.12.2016, p. 6510-6514.

Research output: Contribution to journalArticle

Cho, Youngkyu ; Yoon, Junghyo ; Lim, David Wonbin ; Kim, Jaehoon ; Lee, Jeong Hoon ; Chung, Seok. / Ion concentration polarization for pre-concentration of biological samples without pH change. In: Analyst. 2016 ; Vol. 141, No. 24. pp. 6510-6514.
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