Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

Hyun Hee L. Lee, Soo Yeon Chae, Myung Kook Son, Hugh I. Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalMass Spectrometry Letters
Volume12
Issue number4
DOIs
Publication statusPublished - 2021 Dec

Keywords

  • Glutamine
  • Ion chemistry
  • Ion mobility mass spectrometry
  • Tryptic peptide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy

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