IRF7 promotes glioma cell invasion by inhibiting AGO2 expression

Jun Kyum Kim, Xiong Jin, Seok Won Ham, Seon Yong Lee, Sunyoung Seo, Sung Chan Kim, Sung Hak Kim, Hyunggee Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Interferon regulatory factor 7 (IRF7) is the master transcription factor that plays a pivotal role in the transcriptional activation of type I interferon genes in the inflammatory response. Our previous study revealed that IRF7 is an important regulator of tumor progression via the expression of inflammatory cytokines in glioma. Here, we report that IRF7 promotes glioma invasion and confers resistance to both chemotherapy and radiotherapy by inhibiting expression of argonaute 2 (AGO2), a regulator of microRNA biogenesis. We found that IRF7 and AGO2 expression levels were negatively correlated in patients with glioblastoma multiforme. Ectopic IRF7 expression led to a reduction in AGO2 expression, while depletion of IRF7 resulted in increased AGO2 expression in the LN-229 glioma cell line. In an in vitro invasion assay, IRF7 overexpression enhanced glioma cell invasion. Furthermore, reconstitution of AGO2 expression in IRF7-overexpressing cells led to decreased cell invasion, whereas the reduced invasion due to IRF7 depletion was rescued by AGO2 depletion. In addition, IRF7 induced chemoresistance and radioresistance of glioma cells by diminishing AGO2 expression. Finally, AGO2 depletion alone was sufficient to accelerate glioma cell invasion in vitro and in vivo, indicating that AGO2 regulates cancer cell invasion. Taken together, our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition.

Original languageEnglish
Pages (from-to)5561-5569
Number of pages9
JournalTumor Biology
Volume36
Issue number7
DOIs
Publication statusPublished - 2015 Feb 14

Fingerprint

Interferon Regulatory Factor-7
Glioma
Interferon Type I
Glioblastoma
MicroRNAs

Keywords

  • AGO2
  • Chemoresistance
  • Glioblastoma
  • Invasion
  • IRF7
  • Radioresistance

ASJC Scopus subject areas

  • Cancer Research

Cite this

Kim, J. K., Jin, X., Ham, S. W., Lee, S. Y., Seo, S., Kim, S. C., ... Kim, H. (2015). IRF7 promotes glioma cell invasion by inhibiting AGO2 expression. Tumor Biology, 36(7), 5561-5569. https://doi.org/10.1007/s13277-015-3226-4

IRF7 promotes glioma cell invasion by inhibiting AGO2 expression. / Kim, Jun Kyum; Jin, Xiong; Ham, Seok Won; Lee, Seon Yong; Seo, Sunyoung; Kim, Sung Chan; Kim, Sung Hak; Kim, Hyunggee.

In: Tumor Biology, Vol. 36, No. 7, 14.02.2015, p. 5561-5569.

Research output: Contribution to journalArticle

Kim, JK, Jin, X, Ham, SW, Lee, SY, Seo, S, Kim, SC, Kim, SH & Kim, H 2015, 'IRF7 promotes glioma cell invasion by inhibiting AGO2 expression', Tumor Biology, vol. 36, no. 7, pp. 5561-5569. https://doi.org/10.1007/s13277-015-3226-4
Kim JK, Jin X, Ham SW, Lee SY, Seo S, Kim SC et al. IRF7 promotes glioma cell invasion by inhibiting AGO2 expression. Tumor Biology. 2015 Feb 14;36(7):5561-5569. https://doi.org/10.1007/s13277-015-3226-4
Kim, Jun Kyum ; Jin, Xiong ; Ham, Seok Won ; Lee, Seon Yong ; Seo, Sunyoung ; Kim, Sung Chan ; Kim, Sung Hak ; Kim, Hyunggee. / IRF7 promotes glioma cell invasion by inhibiting AGO2 expression. In: Tumor Biology. 2015 ; Vol. 36, No. 7. pp. 5561-5569.
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