IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a Go signaling pathway

Lesley Emtage, Sonya Aziz-Zaman, Olivia Padovan-Merhar, H. Robert Horvitz, Christopher Fang-Yen, Niels Ringstad

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To identify molecular mechanisms that function in G-protein signaling, we have performed molecular genetic studies of a simple behavior of the nematode Caenorhabditis elegans, egg laying, which is driven by a pair of serotonergic neurons, the hermaphroditespecific neurons (HSNs). The activity of the HSNs is regulated by the Go-coupled receptor EGL-6, which mediates inhibition of the HSNs by neuropeptides. We report here that this inhibition requires one of three inwardly rectifying K+ channels encoded by the C. elegans genome: IRK-1. Using ChannelRhodopsin-2-mediated stimulation of HSNs, we observed roles for egl-6 and irk-1 in regulating the excitability of HSNs. Although irk-1 is required for inhibition of HSNs by EGL-6 signaling, we found that other Go signaling pathways that inhibitHSNsinvolve irk-1 little or not at all. These findings suggest that the neuropeptide receptor EGL-6 regulates the potassium channel IRK-1 via a dedicated pool of Go not involved in other Go-mediated signaling. We conclude that G-protein-coupled receptors that signal through the same G-protein in the same cell might activate distinct effectors and that specific coupling of a G-protein-coupled receptor to its effectors can be determined by factors other than its associated G-proteins.

Original languageEnglish
Pages (from-to)16285-16295
Number of pages11
JournalJournal of Neuroscience
Volume32
Issue number46
DOIs
Publication statusPublished - 2012 Nov 14
Externally publishedYes

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Potassium Channels
Caenorhabditis elegans
Serotonin
Neurons
GTP-Binding Proteins
G-Protein-Coupled Receptors
Neuropeptide Receptors
Inwardly Rectifying Potassium Channel
Serotonergic Neurons
Neuropeptides
Ovum
Inhibition (Psychology)
Molecular Biology
Genome

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a Go signaling pathway. / Emtage, Lesley; Aziz-Zaman, Sonya; Padovan-Merhar, Olivia; Horvitz, H. Robert; Fang-Yen, Christopher; Ringstad, Niels.

In: Journal of Neuroscience, Vol. 32, No. 46, 14.11.2012, p. 16285-16295.

Research output: Contribution to journalArticle

Emtage, L, Aziz-Zaman, S, Padovan-Merhar, O, Horvitz, HR, Fang-Yen, C & Ringstad, N 2012, 'IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a Go signaling pathway', Journal of Neuroscience, vol. 32, no. 46, pp. 16285-16295. https://doi.org/10.1523/JNEUROSCI.2667-12.2012
Emtage, Lesley ; Aziz-Zaman, Sonya ; Padovan-Merhar, Olivia ; Horvitz, H. Robert ; Fang-Yen, Christopher ; Ringstad, Niels. / IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a Go signaling pathway. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 46. pp. 16285-16295.
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