Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett's oesophagus in Korea?

Hyung Joon Yim, Sang Woo Lee, Rok Son Choung, Young Sun Kim, Jin Yong Kim, Hong Sik Lee, Chi Wook Song, Jai Hyun Choi, Young-Tae Bak, Ho Sang Ryu, Jin Hai Hyun, Dae Soo Kim, Chul Hwan Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Cytokeratin 7/20 staining has been reported to be helpful in diagnosing Barrett's oesophagus and gastric intestinal metaplasia. However, this is still a matter of some controversy. Objective: To determine the diagnostic usefulness of cytokeratin 7/20 immunostaining for short-segment Barrett's oesophagus in Korea. Methods: In patients with Barrett's oesophagus, diagnosed endoscopically, at least two biopsy specimens were taken from just below the squamocolumnar junction. If goblet cells were found histologically with alcian blue staining, cytokeratin 7/20 immunohistochemical stains were performed. Intestinal metaplasia at the cardia was diagnosed whenever biopsy specimens taken from within 2 cm below the oesophagogastric junction revealed intestinal metaplasia. Barrett's cytokeratin 7/20 pattern was defined as cytokeratin 20 positivity in only the superficial gland, combined with cytokeratin 7 positivity in both the superficial and deep glands. Results: Barrett's cytokeratin 7/20 pattern was observed in 28 out of 36 cases (77.8%) with short-segment Barrett's oesophagus, 11 out of 28 cases (39.3%) with intestinal metaplasia at the cardia, and nine out of 61 cases (14.8%) with gastric intestinal metaplasia. The sensitivity and specificity of Barrett's cytokeratin 7/20 pattern were 77.8 and 77.5%, respectively. Conclusion: Barrett's cytokeratin 7/20 pattern can be a useful marker for the diagnosis of short-segment Barrett's oesophagus, although the false positive or false negative rate is approximately 25%.

Original languageEnglish
Pages (from-to)611-616
Number of pages6
JournalEuropean Journal of Gastroenterology and Hepatology
Volume17
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Keratin-20
Keratin-7
Barrett Esophagus
Korea
Keratins
Metaplasia
Cardia
Stomach
Staining and Labeling
Biopsy
Esophagogastric Junction
Alcian Blue
Goblet Cells
Coloring Agents
Sensitivity and Specificity

Keywords

  • Barrett's oesophagus
  • Intestinal metaplasia
  • Keratin
  • Stomach

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett's oesophagus in Korea? / Yim, Hyung Joon; Lee, Sang Woo; Choung, Rok Son; Kim, Young Sun; Kim, Jin Yong; Lee, Hong Sik; Song, Chi Wook; Choi, Jai Hyun; Bak, Young-Tae; Ryu, Ho Sang; Hyun, Jin Hai; Kim, Dae Soo; Kim, Chul Hwan.

In: European Journal of Gastroenterology and Hepatology, Vol. 17, No. 6, 01.06.2005, p. 611-616.

Research output: Contribution to journalArticle

Yim, Hyung Joon ; Lee, Sang Woo ; Choung, Rok Son ; Kim, Young Sun ; Kim, Jin Yong ; Lee, Hong Sik ; Song, Chi Wook ; Choi, Jai Hyun ; Bak, Young-Tae ; Ryu, Ho Sang ; Hyun, Jin Hai ; Kim, Dae Soo ; Kim, Chul Hwan. / Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett's oesophagus in Korea?. In: European Journal of Gastroenterology and Hepatology. 2005 ; Vol. 17, No. 6. pp. 611-616.
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title = "Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett's oesophagus in Korea?",
abstract = "Background: Cytokeratin 7/20 staining has been reported to be helpful in diagnosing Barrett's oesophagus and gastric intestinal metaplasia. However, this is still a matter of some controversy. Objective: To determine the diagnostic usefulness of cytokeratin 7/20 immunostaining for short-segment Barrett's oesophagus in Korea. Methods: In patients with Barrett's oesophagus, diagnosed endoscopically, at least two biopsy specimens were taken from just below the squamocolumnar junction. If goblet cells were found histologically with alcian blue staining, cytokeratin 7/20 immunohistochemical stains were performed. Intestinal metaplasia at the cardia was diagnosed whenever biopsy specimens taken from within 2 cm below the oesophagogastric junction revealed intestinal metaplasia. Barrett's cytokeratin 7/20 pattern was defined as cytokeratin 20 positivity in only the superficial gland, combined with cytokeratin 7 positivity in both the superficial and deep glands. Results: Barrett's cytokeratin 7/20 pattern was observed in 28 out of 36 cases (77.8{\%}) with short-segment Barrett's oesophagus, 11 out of 28 cases (39.3{\%}) with intestinal metaplasia at the cardia, and nine out of 61 cases (14.8{\%}) with gastric intestinal metaplasia. The sensitivity and specificity of Barrett's cytokeratin 7/20 pattern were 77.8 and 77.5{\%}, respectively. Conclusion: Barrett's cytokeratin 7/20 pattern can be a useful marker for the diagnosis of short-segment Barrett's oesophagus, although the false positive or false negative rate is approximately 25{\%}.",
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author = "Yim, {Hyung Joon} and Lee, {Sang Woo} and Choung, {Rok Son} and Kim, {Young Sun} and Kim, {Jin Yong} and Lee, {Hong Sik} and Song, {Chi Wook} and Choi, {Jai Hyun} and Young-Tae Bak and Ryu, {Ho Sang} and Hyun, {Jin Hai} and Kim, {Dae Soo} and Kim, {Chul Hwan}",
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T1 - Is cytokeratin immunoreactivity useful in the diagnosis of short-segment Barrett's oesophagus in Korea?

AU - Yim, Hyung Joon

AU - Lee, Sang Woo

AU - Choung, Rok Son

AU - Kim, Young Sun

AU - Kim, Jin Yong

AU - Lee, Hong Sik

AU - Song, Chi Wook

AU - Choi, Jai Hyun

AU - Bak, Young-Tae

AU - Ryu, Ho Sang

AU - Hyun, Jin Hai

AU - Kim, Dae Soo

AU - Kim, Chul Hwan

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Background: Cytokeratin 7/20 staining has been reported to be helpful in diagnosing Barrett's oesophagus and gastric intestinal metaplasia. However, this is still a matter of some controversy. Objective: To determine the diagnostic usefulness of cytokeratin 7/20 immunostaining for short-segment Barrett's oesophagus in Korea. Methods: In patients with Barrett's oesophagus, diagnosed endoscopically, at least two biopsy specimens were taken from just below the squamocolumnar junction. If goblet cells were found histologically with alcian blue staining, cytokeratin 7/20 immunohistochemical stains were performed. Intestinal metaplasia at the cardia was diagnosed whenever biopsy specimens taken from within 2 cm below the oesophagogastric junction revealed intestinal metaplasia. Barrett's cytokeratin 7/20 pattern was defined as cytokeratin 20 positivity in only the superficial gland, combined with cytokeratin 7 positivity in both the superficial and deep glands. Results: Barrett's cytokeratin 7/20 pattern was observed in 28 out of 36 cases (77.8%) with short-segment Barrett's oesophagus, 11 out of 28 cases (39.3%) with intestinal metaplasia at the cardia, and nine out of 61 cases (14.8%) with gastric intestinal metaplasia. The sensitivity and specificity of Barrett's cytokeratin 7/20 pattern were 77.8 and 77.5%, respectively. Conclusion: Barrett's cytokeratin 7/20 pattern can be a useful marker for the diagnosis of short-segment Barrett's oesophagus, although the false positive or false negative rate is approximately 25%.

AB - Background: Cytokeratin 7/20 staining has been reported to be helpful in diagnosing Barrett's oesophagus and gastric intestinal metaplasia. However, this is still a matter of some controversy. Objective: To determine the diagnostic usefulness of cytokeratin 7/20 immunostaining for short-segment Barrett's oesophagus in Korea. Methods: In patients with Barrett's oesophagus, diagnosed endoscopically, at least two biopsy specimens were taken from just below the squamocolumnar junction. If goblet cells were found histologically with alcian blue staining, cytokeratin 7/20 immunohistochemical stains were performed. Intestinal metaplasia at the cardia was diagnosed whenever biopsy specimens taken from within 2 cm below the oesophagogastric junction revealed intestinal metaplasia. Barrett's cytokeratin 7/20 pattern was defined as cytokeratin 20 positivity in only the superficial gland, combined with cytokeratin 7 positivity in both the superficial and deep glands. Results: Barrett's cytokeratin 7/20 pattern was observed in 28 out of 36 cases (77.8%) with short-segment Barrett's oesophagus, 11 out of 28 cases (39.3%) with intestinal metaplasia at the cardia, and nine out of 61 cases (14.8%) with gastric intestinal metaplasia. The sensitivity and specificity of Barrett's cytokeratin 7/20 pattern were 77.8 and 77.5%, respectively. Conclusion: Barrett's cytokeratin 7/20 pattern can be a useful marker for the diagnosis of short-segment Barrett's oesophagus, although the false positive or false negative rate is approximately 25%.

KW - Barrett's oesophagus

KW - Intestinal metaplasia

KW - Keratin

KW - Stomach

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U2 - 10.1097/00042737-200506000-00004

DO - 10.1097/00042737-200506000-00004

M3 - Article

VL - 17

SP - 611

EP - 616

JO - European Journal of Gastroenterology and Hepatology

JF - European Journal of Gastroenterology and Hepatology

SN - 0954-691X

IS - 6

ER -