Ischemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage

Koo Hwang In, Ki Yeon Yoo, Won Kim Dae, Young Kim So, Hong Park Jun, Young Ryoo Zae, Joon Kim, Young Choi Soo, Moo Ho Won

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Some ribosomal proteins are important regulators of development and DNA repair. However, few studies have been conducted on ribosomal protein S3 (rpS3) in the ischemic hippocampus. In the present study, we investigated ischemia-induced changes in rpS3 immunoreactivity, rpS3 mRNA, and protein levels in the hippocampal CA1 region of Mongolian gerbil after 5 min of transient forebrain ischemia. RpS3 immunoreactivity and its protein level were found to be significantly elevated at 6 hr after ischemia/reperfusion and then continuously decreased with time. RT-PCR analysis also showed that rpS3 mRNA levels were significantly elevated in CA1 at 6 hr after transient ischemia. In addition, during the course of this study, we developed a delivery vector (Pep-1) and its rpS3 fusion protein (Pep-1-rpS3) to elucidate the role of rpS3 in ischemia-induced damage. Pep-1-rpS3 administration to ischemic animals significantly and dose dependently increased neuronal survival in the stratum pyramidale of CA1. Moreover, Pep-1-rpS3 treatment reduced terminal deoxynucleotidyl dUTP nick-end labeling-positive CA1 pyramidal cell numbers in the stratum pyramidale. To elucidate how Pep-1-rpS3 ameliorates ischemic damage, changes in 4-hydroxy-2-nonnenal (HNE; an indicator of lipid peroxidation) immunoreactivity and protein levels were investigated. HNE levels and immunoreactivities in Pep-1-rpS3-treated ischemic animals were lower than in corresponding Pep-1-treated ischemic animals. These results indicate that rpS3 has a neuroprotective effect in the brain exposed to ischemia.

Original languageEnglish
Pages (from-to)1823-1835
Number of pages13
JournalJournal of Neuroscience Research
Volume86
Issue number8
DOIs
Publication statusPublished - 2008 Jun 26

Fingerprint

Neuroprotective Agents
Ischemia
Proteins
Hippocampal CA1 Region
Messenger RNA
ribosomal protein S3
Gerbillinae
Pyramidal Cells
Ribosomal Proteins
Prosencephalon
DNA Repair
Lipid Peroxidation
Reperfusion
Hippocampus
Cell Count
Polymerase Chain Reaction
Brain

Keywords

  • CA1 pyramidal cells
  • Neuroprotection
  • Pep-1 vector
  • Ribosomal protein S3
  • Transient forebrain ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ischemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage. / In, Koo Hwang; Yoo, Ki Yeon; Dae, Won Kim; So, Young Kim; Jun, Hong Park; Zae, Young Ryoo; Kim, Joon; Soo, Young Choi; Won, Moo Ho.

In: Journal of Neuroscience Research, Vol. 86, No. 8, 26.06.2008, p. 1823-1835.

Research output: Contribution to journalArticle

In, Koo Hwang ; Yoo, Ki Yeon ; Dae, Won Kim ; So, Young Kim ; Jun, Hong Park ; Zae, Young Ryoo ; Kim, Joon ; Soo, Young Choi ; Won, Moo Ho. / Ischemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 8. pp. 1823-1835.
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AU - Jun, Hong Park

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AB - Some ribosomal proteins are important regulators of development and DNA repair. However, few studies have been conducted on ribosomal protein S3 (rpS3) in the ischemic hippocampus. In the present study, we investigated ischemia-induced changes in rpS3 immunoreactivity, rpS3 mRNA, and protein levels in the hippocampal CA1 region of Mongolian gerbil after 5 min of transient forebrain ischemia. RpS3 immunoreactivity and its protein level were found to be significantly elevated at 6 hr after ischemia/reperfusion and then continuously decreased with time. RT-PCR analysis also showed that rpS3 mRNA levels were significantly elevated in CA1 at 6 hr after transient ischemia. In addition, during the course of this study, we developed a delivery vector (Pep-1) and its rpS3 fusion protein (Pep-1-rpS3) to elucidate the role of rpS3 in ischemia-induced damage. Pep-1-rpS3 administration to ischemic animals significantly and dose dependently increased neuronal survival in the stratum pyramidale of CA1. Moreover, Pep-1-rpS3 treatment reduced terminal deoxynucleotidyl dUTP nick-end labeling-positive CA1 pyramidal cell numbers in the stratum pyramidale. To elucidate how Pep-1-rpS3 ameliorates ischemic damage, changes in 4-hydroxy-2-nonnenal (HNE; an indicator of lipid peroxidation) immunoreactivity and protein levels were investigated. HNE levels and immunoreactivities in Pep-1-rpS3-treated ischemic animals were lower than in corresponding Pep-1-treated ischemic animals. These results indicate that rpS3 has a neuroprotective effect in the brain exposed to ischemia.

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KW - Transient forebrain ischemia

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