Abstract
Although isobutanol has been successfully produced from glucose-based media by various microbes, only a few studies have reported isobutanol production from lignocellulosic biomass. In this study, the feasibility of producing isobutanol from hydrothermally-pretreated empty fruit bunches (EFBs) was analyzed. Firstly, the Escherichia coli JK209 strain was constructed; subsequently, optimal fermentation conditions for isobutanol production using this strain were determined. Next, the raw EFBs were hydrothermally pretreated at 190 °C for 15 min, and the pretreated EFBs were hydrolyzed using 10 FPU of cellulase/g glucan. Finally, the resulting hydrolysate was fermented at 25 °C for 84 h, and isobutanol give rise to the yield of 0.26 g isobutanol/g glucose. These results imply that 80.1 g of isobutanol could be produced from 1 kg dry weight of EFBs. To our knowledge, this is the first feasibility study of isobutanol production from pretreated EFBs.
Original language | English |
---|---|
Pages (from-to) | 1124-1130 |
Number of pages | 7 |
Journal | Renewable Energy |
Volume | 157 |
DOIs | |
Publication status | Published - 2020 Sep |
Keywords
- Empty fruit bunches
- Isobutanol
- Lignocellulose
- Pretreatment
- Separate hydrolysis and fermentation
ASJC Scopus subject areas
- Renewable Energy, Sustainability and the Environment