Isobutanol production from empty fruit bunches

Jungwoo Yang, Jae Kyun Kim, Jung Oh Ahn, Young Ha Song, Chul Soo Shin, Yong Cheol Park, Kyoung Heon Kim

Research output: Contribution to journalArticle

Abstract

Although isobutanol has been successfully produced from glucose-based media by various microbes, only a few studies have reported isobutanol production from lignocellulosic biomass. In this study, the feasibility of producing isobutanol from hydrothermally-pretreated empty fruit bunches (EFBs) was analyzed. Firstly, the Escherichia coli JK209 strain was constructed; subsequently, optimal fermentation conditions for isobutanol production using this strain were determined. Next, the raw EFBs were hydrothermally pretreated at 190 °C for 15 min, and the pretreated EFBs were hydrolyzed using 10 FPU of cellulase/g glucan. Finally, the resulting hydrolysate was fermented at 25 °C for 84 h, and isobutanol give rise to the yield of 0.26 g isobutanol/g glucose. These results imply that 80.1 g of isobutanol could be produced from 1 kg dry weight of EFBs. To our knowledge, this is the first feasibility study of isobutanol production from pretreated EFBs.

Original languageEnglish
Pages (from-to)1124-1130
Number of pages7
JournalRenewable Energy
Volume157
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Empty fruit bunches
  • Isobutanol
  • Lignocellulose
  • Pretreatment
  • Separate hydrolysis and fermentation

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

Cite this

Yang, J., Kim, J. K., Ahn, J. O., Song, Y. H., Shin, C. S., Park, Y. C., & Kim, K. H. (2020). Isobutanol production from empty fruit bunches. Renewable Energy, 157, 1124-1130. https://doi.org/10.1016/j.renene.2020.05.067