Isoflavones inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro

Jang Hoon Sung, Sung-Joon Lee, Kwan Hwa Park, Tae Wha Moon

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Isoflavones identified as inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in soybean paste were assayed using the catalytic portion of Syrian hamster HMG-CoA reductase, and the kinetic values were measured using HMG-CoA and NADPH. The inhibition of HMG-CoA reductase by these inhibitors was competitive with HMG-CoA and noncompetitive with NADPH. Ki values for genistein, daidzein, and glycitein were 27.7, 49.5, and 94.7 μM, respectively.

Original languageEnglish
Pages (from-to)428-432
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number2
DOIs
Publication statusPublished - 2004 Feb 1
Externally publishedYes

Fingerprint

Isoflavones
coenzyme A
Coenzyme A
isoflavones
Oxidoreductases
NADP
NADP (coenzyme)
glycitein
daidzein
Genistein
Mesocricetus
genistein
Ointments
Soybeans
soybeans
kinetics
Kinetics
In Vitro Techniques
3-hydroxy-3-methylglutaryl-coenzyme A
hydroxymethylglutaryl-CoA reductases

Keywords

  • Cholesterol
  • Genistein
  • HMG-CoA reductase
  • Isoflavone
  • Soybean

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Isoflavones inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro. / Sung, Jang Hoon; Lee, Sung-Joon; Park, Kwan Hwa; Moon, Tae Wha.

In: Bioscience, Biotechnology and Biochemistry, Vol. 68, No. 2, 01.02.2004, p. 428-432.

Research output: Contribution to journalArticle

Sung, Jang Hoon ; Lee, Sung-Joon ; Park, Kwan Hwa ; Moon, Tae Wha. / Isoflavones inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro. In: Bioscience, Biotechnology and Biochemistry. 2004 ; Vol. 68, No. 2. pp. 428-432.
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