Isolation and characterization of chorionic mesenchymal stromal cells from human full term placenta

Bo Kyung Koo, In Yang Park, Jiyeon Kim, Ji Hyun Kim, Ahlm Kwon, Myungshin Kim, Yonggoo Kim, Jong Chul Shin, Jong-Hoon Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study focused on the characterization of mesenchymal stromal cells (MSCs) from the chorion of human full term placenta from 15 donors. Chorionic MSCs revealed homologous fibroblast-like morphology and expressed CD73, CD29, CD105, and CD90. The hematopoietic stem cell markers including HLA DR, CD11b, CD34, CD79a, and CD45 were not expressed. The growth kinetics of their serial passage was steady at the later passages (passage 10). The multilineage capability of chorionic MSCs was demonstrated by successful adipogenic, osteogenic and chondrogenic differentiation and associated gene expression. Chorionic MSCs expressed genes associated with undifferentiated cells (NANOG, OCT4, REX1) and cardiogenic or neurogenic markers such as SOX2, FGF4, NES, MAP2, and NF. TERT was negative in all the samples. These findings suggest that chorionic MSCs undifferentiated stem cells and less likely to be transformed into cancer cells. A low HLA DR expression suggests that chorionic MSCs may serve as a great source of stem cells for transplantation because of their immune-privileged status and their immunosuppressive effect. Based on these unique properties, it is concluded that chorionic MSCs are pluripotent stem cells that are probably less differentiated than BM-MSCs, and they have considerable potential for use in cell-based therapies.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalJournal of Korean Medical Science
Volume27
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1

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Mesenchymal Stromal Cells
Placenta
HLA-DR Antigens
Serial Passage
Chorion
Pluripotent Stem Cells
Stem Cell Transplantation
Immunosuppressive Agents
Cell- and Tissue-Based Therapy
Hematopoietic Stem Cells
Stem Cells
Fibroblasts
Gene Expression
Growth
Genes
Neoplasms

Keywords

  • Chorion
  • Gene expression
  • Human placenta
  • Mesenchymal stromal cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Isolation and characterization of chorionic mesenchymal stromal cells from human full term placenta. / Koo, Bo Kyung; Park, In Yang; Kim, Jiyeon; Kim, Ji Hyun; Kwon, Ahlm; Kim, Myungshin; Kim, Yonggoo; Shin, Jong Chul; Kim, Jong-Hoon.

In: Journal of Korean Medical Science, Vol. 27, No. 8, 01.08.2012, p. 857-863.

Research output: Contribution to journalArticle

Koo, Bo Kyung ; Park, In Yang ; Kim, Jiyeon ; Kim, Ji Hyun ; Kwon, Ahlm ; Kim, Myungshin ; Kim, Yonggoo ; Shin, Jong Chul ; Kim, Jong-Hoon. / Isolation and characterization of chorionic mesenchymal stromal cells from human full term placenta. In: Journal of Korean Medical Science. 2012 ; Vol. 27, No. 8. pp. 857-863.
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